Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
13 Feb- 12 Mar 1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Trade name, Glycerolmonotalloate
- Analytical purity: 100%
- Lot/batch No.: v.3.11.95
- Expiration date of the lot/batch: 1997-11-03
- Stability under test conditions: 2 years
- Water solubility (under test conditions): non soluble

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum: Activated sludge from the sewage treatment plant Hochdahl, Germany
- Concentration of sludge: 1 drop sewage effluent/L cultivation solution
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
98.27 mg/L
Based on:
test mat.
Initial conc.:
99.4 mg/L
Based on:
test mat.
Initial conc.:
100.03 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: Mineral nutrient solution
- Test temperature: 22 ± 1 °C

TEST SYSTEM
- Culturing apparatus: glass bottle
- Number of culture flasks/concentration: 3

SAMPLING
- Sampling frequency: daily from day 0 - day 28
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
54
St. dev.:
9.1
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
71
St. dev.:
10.2
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
77
St. dev.:
9.3
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
79
St. dev.:
8.7
Sampling time:
28 d
Details on results:
The test substance reached 79% biodegradation after 28 days, fulfilling the 10-day window criterion. Therefore, it is considered readily biodegradable

BOD5 / COD results

Results with reference substance:
The reference substance reached 80% biodegradation within 14 days, fulfilling the validity criterion (>60%)

Any other information on results incl. tables

Table 1:  Biodegradation (%BOD/ThOD) of Test Substance (TS) and Reference Substance (RS)

 

Biodegradation (%BOD/ThOD) of Test Substance (TS) and Reference Substance (RS)

 

Day

TS I

TS II

TS IIII

TS

Mean value

RS I

RS II

RS IIII

RS

Mean value

0

0

0

0

0

0

0

0

0

1

9

2

5

5

15

12

23

17

2

31

14

16

20

53

52

52

52

3

29

21

21

27

59

57

56

57

4

52

31

30

38

66

66

63

65

5

57

39

37

45

71

70

65

69

6

61

46

42

50

74

73

67

71

7

64

53

46

54

76

75

70

74

8

67

58

49

58

78

76

73

76

9

70

62

51

61

78

77

76

77

10

73

66

53

64

79

78

76

78

11

75

69

55

66

79

79

76

78

12

76

71

57

68

80

80

77

79

13

77

74

58

70

81

81

77

80

14

78

75

59

71

81

81

77

80

15

79

77

61

72

82

81

78

81

16

80

78

62

73

82

82

78

81

17

81

79

63

74

83

82

79

81

18

81

80

64

75

84

83

79

82

19

82

81

65

76

84

83

79

82

20

82

81

65

76

84

83

79

82

21

83

81

66

77

84

83

79

82

22

83

82

66

77

85

84

79

83

23

84

82

67

77

85

84

80

83

24

84

82

67

78

84

84

80

83

25

85

83

68

78

85

84

80

83

26

85

83

68

79

85

85

81

84

27

85

83

69

79

86

85

80

84

28

85

83

69

79

86

85

80

84

pH values are not reported.

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable