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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1998
Reference Type:
secondary source
Title:
Chemical Risk Information Platform- Physical Chemical data
Author:
NITE
Year:
2009
Bibliographic source:
http://www.safe.nite.go.jp

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzothiazole
EC Number:
202-396-2
EC Name:
Benzothiazole
Cas Number:
95-16-9
Molecular formula:
C7H5NS
IUPAC Name:
benzothiazole
Details on test material:
- Name of test material (as cited in study report): benzothiazole
- Analytical purity: 98.1 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
- Controls: yes (dilution water)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Feeding during test: no

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
20.4 °C
pH:
8.1 - 8.3
Dissolved oxygen:
8.2 - 8.4 mg/L (48 h)
Nominal and measured concentrations:
nominal: 10, 18, 32, 56 and 100 mg/L
measured: 6.9, 13, 23, 41 and 73 mg/L (geometric mean of initial and end concentration)
Details on test conditions:
TEST SYSTEM
- Test volume: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: de-chlorinated tap water
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
19 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 16 - 23
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
6.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- EC50 (48h) = 0.62 mg/L

Any other information on results incl. tables

The lose of test substance throughout the test was approx. 36%.

Applicant's summary and conclusion

Executive summary:

MOE (1997):

Japanese Ministry of the Environment conducted a test on acute toxicity to Daphnia magna according to OECD TG 202. After an exposure period of 48 hours a measured EC50 of 19 mg/L based on mobility was found.