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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1997
Report Date:
1998
Reference Type:
secondary source
Title:
Chemical Risk Information Platform- Physical Chemical data
Author:
NITE
Year:
2009
Bibliographic source:
http://www.safe.nite.go.jp

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): benzothiazole
- Analytical purity: 98.1 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
- Controls: yes (dilution water)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Feeding during test: no

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
20.4 °C
pH:
8.1 - 8.3
Dissolved oxygen:
8.2 - 8.4 mg/L (48 h)
Nominal and measured concentrations:
nominal: 10, 18, 32, 56 and 100 mg/L
measured: 6.9, 13, 23, 41 and 73 mg/L (geometric mean of initial and end concentration)
Details on test conditions:
TEST SYSTEM
- Test volume: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: de-chlorinated tap water
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
19 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 16 - 23
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
6.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- EC50 (48h) = 0.62 mg/L

Any other information on results incl. tables

The lose of test substance throughout the test was approx. 36%.

Applicant's summary and conclusion

Executive summary:

MOE (1997):

Japanese Ministry of the Environment conducted a test on acute toxicity to Daphnia magna according to OECD TG 202. After an exposure period of 48 hours a measured EC50 of 19 mg/L based on mobility was found.