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EC number: 225-306-3 | CAS number: 4767-03-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
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- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
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- Toxicological Summary
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Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 August 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guideline compliant study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2-bis(hydroxymethyl)propionic acid (Bis-MPA)
- IUPAC Name:
- 2,2-bis(hydroxymethyl)propionic acid (Bis-MPA)
- Reference substance name:
- 2,2-bis(hydroxymethyl)propionic acid
- EC Number:
- 225-306-3
- EC Name:
- 2,2-bis(hydroxymethyl)propionic acid
- Cas Number:
- 4767-03-7
- Molecular formula:
- C5H10O4
- IUPAC Name:
- 3-hydroxy-2-(hydroxymethyl)-2-methylpropanoic acid
- Details on test material:
- - Name of test material (as cited in study report): Dimethylolpropionsaure (Bis-MPA)
- Physical state: white solid
- Analytical purity: 99.4%
- Lot/batch No.: SE 8069
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- not required
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge was taken from laboratory wastewater plants treating municipal sewage.
The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated for about 24 hours. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- Not applicable
- Test temperature:
- 20 ± 2 °C
- pH:
- See results tables
- Dissolved oxygen:
- See results tables
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentration of dimethylolpropionsaure used: 1000 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Not applicable
- Results with reference substance (positive control):
- Reference substance after 30 min incubation time:
EC20(mg/L) - 1.5
EC50(mg/L) - 5.5
EC80(mg/L) - 45
- Reported statistics and error estimates:
- Not aplicable
Any other information on results incl. tables
pH values during the study
Vessel number |
BC1 |
BC2 |
BC3 |
TS1 |
pH value before adding the inoculum: |
|
|
|
|
pH value after 30 minute incubation |
- |
- |
- |
7.5 |
Results of the definitive test after 30 min incubation period with 1000 mg/L dimethylolpropionsaure
Vessel no. |
mv BC |
TS1 |
RS1 |
RS2 |
RS3 |
Oxygen concentration start (mg O2/L) |
- |
7.8 |
7.4 |
8.2 |
8.7 |
Oxygen concentration after 6 min (mg O2/L) |
- |
5.8 |
5.7 |
7.5 |
8.5 |
Oxygen consumption rate (mg O2/L x 6 min) |
1.9 |
2.0 |
1.7 |
0.7 |
0.2 |
Oxygen consumption rate (mg O2/L x h) |
19 |
20 |
17 |
7 |
2 |
Specific oxygen consumption rate (mg O2/g x h) |
19 |
20 |
17 |
7 |
2 |
Change in oxygen consumption rate compared with the blank control (%) |
- |
-5 |
11 |
63 |
89 |
mv BC = mean value of blank control
TS = test substance
RS = reference number
Reference substance after 30 min incubation time
|
EC20(mg/L) |
EC50(mg/L) |
EC80(mg/L) |
EC20(mg/L) |
Reference substance |
ca. 1.5 |
ca. 5.5 |
ca. 45 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The EC50 for the reference inhibitor, 3,5-dichlorophenol was in the range of 5 - 30 mg/L.
- Conclusions:
- Activated sludge obtained from laboratory wastewater plants treating municipal sewage was exposed in a 30 -minute inhibition of oxygen consumption test to 1000 mg dimethylolpropionsaure/L. The test design followed OECD 209 guidelines. The 30 -minute EC50 based on respiration inhibition was >1000 mg/L (the highest nominal exposure concentration tested).
- Executive summary:
Activated sludge obtained from laboratory wastewater plants treating municipal sewage was exposed in a 30 -minute inhibition of oxygen consumption test to 1000 mg dimethylolpropionsaure/L. The test design followed OECD 209 guidelines. The 30 -minute EC50 based on respiration inhibition was >1000 mg/L (the highest nominal exposure concentration tested).
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