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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999-3-12 - 1999-04-09
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
Specific details on test material used for the study:
- Name of the test material used in the study report: Dimethylolpropionsäure, 2,2-Bis(hydroxymethyl)propansäure
- Batch no.: SE 8069
- Purity: 99.4%
- Storage conditions: storage at room temperature
Oxygen conditions:
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Activated sludge from laboratory waste water plants treating municipal and synthetic sewage. Concentration of the dry matter was 30 mg/L.
Duration of test (contact time):
28 d
Initial conc.:
42 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
- Composition of medium: Activated sludge from laboratory waste water plants
- Additional substrate: None
- Solubilising agent (type and concentration if used): not required
- Test temperature: No data
- pH: No data
- pH adjusted: No data
- CEC (meq/100 g): No data
- Aeration of dilution water: No data
- Suspended solids concentration: 30 mg/L
- Continuous darkness: No data

Reference substance:
Preliminary study:
No data available
Test performance:
No data available
% degradation (DOC removal)
90 - 100
Sampling time:
28 d
Details on results:
Over the ten day window from day 10 to day 20 DOC removal of the test substance increased from approximately 10% on day 10 to greater than 90% by day 20.
Results with reference substance:
DOC removal of the test substance (aniline) was rapid with greater than 90% removal being observed by day 5 of the study.

After an initial period of equilibration DOC removal for the test substance was rapid and efficient. Over the ten day period between day ten and day twenty DOC removal moved from a mean of 10% to over 90%. This indicates the substance has reached the pass criterium for ready biodegradability and can be considered fully and readily biodegradable.

Validity criteria fulfilled:
Interpretation of results:
readily biodegradable
The substance is readily biodegradable.
Executive summary:

In a study conducted to guideline OECD 301A, Bis-MPA was 90 -100% biodegradaded over 28 days (i.e. >70%). During the 10-day window removal of DOC increased from 10% to over 90%. The substance Bis-MPA is therefore readily biodegradable and biological removal in the environment can be considered to be complete and rapid.

Description of key information

The substance Bis-MPA is readily biodegradable in a study conducted to OECD guideline 301A.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:

Additional information

A classification of readily biodegradable means that Bis-MPA will be rapidly and effectively removed from the environmental compartments by biological degradation. As such removal from the STP is expected to be highly efficient and half lives in soil and sediment will be short. Thus, the likelihood of long-term residence in the environment is considered to be negligible for Bis-MPA.