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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Evaluation of the V79 cell metabolic co-operation assay as a screen in vitro for developmental toxicants
Author:
Toraason M, Bohrman JS, and Krieg E
Year:
1992
Bibliographic source:
Toxic. in Vitro 6, 165-174

Materials and methods

Principles of method if other than guideline:
Phthalimide was tested for its effect on intercellular communication in the V79 cell metabolic co-operation assay (as inhibition of intercellular communication is proposed to be one of several possible mechanisms of teratogenesis, the V79 cell metabolic co-operation assay is evaluated as a in vitro screen for developmental toxicants).
GLP compliance:
not specified
Type of method:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
Phthalimide
EC Number:
201-603-3
EC Name:
Phthalimide
Cas Number:
85-41-6
Molecular formula:
C8H5NO2
IUPAC Name:
1H-isoindole-1,3(2H)-dione
Details on test material:
- Name of test material (as cited in study report): Phthalimide
- Analytical purity: no data

Test animals

Species:
other: V79 cell

Administration / exposure

Vehicle:
DMSO
Analytical verification of doses or concentrations:
no
Doses / concentrations
Remarks:
Doses / Concentrations:
25, 50, 100, 150, and 200 µg/ml
Basis:
nominal conc.
Control animals:
other: not applicable

Examinations

Positive control:
the positive control 12-O-tetradecanoylphorbol-13-acetate (TPA) at 4ng/mL

Results and discussion

Details on results:
No cytotoxic effects of phthalimide were observed at any concentration. Phthalimide did not inhibit metabolic co-operation of the V79 cells.

Any other information on results incl. tables

Compound             Cytotoxicity          metabolic
concentration
           assay              cooperation assay
  [µg/ml]
------------------------------------------------------------------

DMSO
                       100 ± 12                   19 ± 5
TPA
                         102 ± 14                  106 ± 11
25
                           96 ± 8                     22 ± 4
50
                          104 ± 10                    23 ± 5
100
                          96 ± 8                     23 ± 6
150
                         100 ± 10                    24 ± 5
200
                          99 ± 5                     22 ± 6


Applicant's summary and conclusion