Phthalimide

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Evaluation of the V79 cell metabolic co-operation assay as a screen in vitro for developmental toxicants

Author:

Toraason M, Bohrman JS, and Krieg E

Year:

1992

Bibliographic source:

Toxic. in Vitro 6, 165-174

Materials and methods

Principles of method if other than guideline:

Phthalimide was tested for its effect on intercellular communication in the V79 cell metabolic co-operation assay (as inhibition of intercellular communication is proposed to be one of several possible mechanisms of teratogenesis, the V79 cell metabolic co-operation assay is evaluated as a in vitro screen for developmental toxicants).

GLP compliance:

not specified

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Phthalimide

EC Number:

201-603-3

EC Name:

Phthalimide

Cas Number:

85-41-6

Molecular formula:

C8H5NO2

IUPAC Name:

1H-isoindole-1,3(2H)-dione

Details on test material:

- Name of test material (as cited in study report): Phthalimide
- Analytical purity: no data

Test animals

Species:

other: V79 cell

Administration / exposure

Vehicle:

DMSO

Analytical verification of doses or concentrations:

no

Doses / concentrations

Remarks:

Doses / Concentrations:
25, 50, 100, 150, and 200 µg/ml
Basis:
nominal conc.

Control animals:

other: not applicable

Examinations

Positive control:

the positive control 12-O-tetradecanoylphorbol-13-acetate (TPA) at 4ng/mL

Results and discussion

Details on results:

No cytotoxic effects of phthalimide were observed at any concentration. Phthalimide did not inhibit metabolic co-operation of the V79 cells.

Any other information on results incl. tables

Compound             Cytotoxicity          metabolic
concentration           assay              cooperation assay
  [µg/ml]
------------------------------------------------------------------

DMSO                       100 ± 12                   19 ± 5
TPA                         102 ± 14                  106 ± 11
25                           96 ± 8                     22 ± 4
50                          104 ± 10                    23 ± 5
100                          96 ± 8                     23 ± 6
150                         100 ± 10                    24 ± 5
200                          99 ± 5                     22 ± 6