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Short-term toxicity to aquatic invertebrates

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Description of key information

No short-term toxicity in the classifiable range of CLP

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
41 mg/L

Additional information

The short-term toxicity of Guanidine carbonate (CAS 593-85-1) to aquatic invertebrates was investigated in a GLP-compliant study (Fenzl 1998) using daphnids (Daphnia magna) according to the EU C.1 (1992) protocol. The experiment is deemed valid, conclusive and thus suitable for assessment without restrictions.

Twenty daphnids (4 replicates of 5 animals) were exposed to nominal test item concentrations of 0, 10, 18, 32, 56 and 100 mg/L. Mean measured concentrations (two replicates) were determined at the two highest concentrations and analytically 96 to 104 % of nominal were verified. Thus the effects were related to the nominal concentrations. Immobilisation and animals trapped at the surface were recorded after 24 and 48 h.

Effects were observed from 32 mg/L on. After 48-h 25 % of the test animals were recorded immobile at this level, 80 % at 56 mg/L and 100 % (all 20 daphnids) at 100 mg/L. Accordingly the acute toxicity of the test item to the freshwater invertebrates gave a 48-h EC 50 of 41 mg/L with 95 % confidence limits of 35 and 48 mg/L. No animals were trapped at the surface.

In conclusion the test item was found not toxic to aquatic invertebrates up to the relevant levels considered for acute hazard to the aquatic life in the EU (i.e. 10 mg/L according to CLP, Regulation (EC) No 1272/2008) and no aquatic hazard classification is required the results of this study.

The study of van der Schalie (1985), used to read-across the long-term effects revealed a 48-h EC50 of the source chemical 70.2 mg Guanidine mononitrate/L, which delivers on the basis of equimolar conversion to the target chemical in 51.8 mg Guanidine carbonate/L. This supports the applicability of the employed approach.

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