Registration Dossier
Registration Dossier
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EC number: 200-618-2 | CAS number: 65-85-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation / corrosion, other
- Remarks:
- Study predates requirements to perform in vitro screening ahead of in vivo studies.
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Limited study details published. Not GLP. Despite its reliability score of K2, this study is considered as Key study (in favour over other K1 studies) on the grounds that the Guinea-pig response is considered as more consistent with human responses.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Groups of ten adult female guinea pigs, rats, and mice were used for each challenge. The thicknesses of the earlobes were measured.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Benzoic acid
- EC Number:
- 200-618-2
- EC Name:
- Benzoic acid
- Cas Number:
- 65-85-0
- Molecular formula:
- C7H6O2
- IUPAC Name:
- benzoic acid
- Details on test material:
- Batch No.: not specifiedPurity: not specified
Constituent 1
Test animals
- Species:
- other: guinea pig, rat and mouse
- Strain:
- other: Hartley, Sprague-Dawley and ICR
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS- Source: Guinea pigs from Charles River Breeding Laboratories, Wilmington, MA; rats from Bantin & Kingman, Inc., Fremont, CA; mice from Simonsen Laboratories, Inc., Gilroy, CA.- Age at study initiation: adult- Weight at study initiation: Guinea pigs weight 350-500 gm, rats weight 180-210 gm.No additional data
Test system
- Type of coverage:
- not specified
- Preparation of test site:
- not specified
- Vehicle:
- other: ethyl alcohol
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL- Amount(s) applied (volume or weight with unit): guinea pig: 50 μL; rat: 20 μL; mouse: 5 μL.- Concentration (if solution): 20%No additional data
- Duration of treatment / exposure:
- 3 hours
- Observation period:
- 3 hours
- Number of animals:
- 10 females
- Details on study design:
- The thicknesses of the earlobes were measured three times before and every 10 minutes during the first hour, every 15 minutes during the second hour, and every 30 minutes during the third hour after application of the test substances, using a micrometer with round touching plates 6 mm in diameter. The micrometer plates were placed on the mid part of the earlobe, 1 mm from the outer rim in rat and mouse and 7 mm, in guinea pig. The means of the three measurements were used. Statistical significance was determined by Student's t test.
Results and discussion
In vivo
Results
- Remarks on result:
- other: In the guinea pig the test substance produced erythema and swelling of the earlobe. The test substance did not produce reactions in the mouse and rat ear.
- Irritant / corrosive response data:
- In the guinea pig the test substance produced erythema and swelling of the earlobe. Maximal swelling was observed at 20 to 50 minutes after application and was then seen to decrease progressively during the observation period of 3 hours. The test substance did not produce reactions in the mouse and rat ear.
- Other effects:
- No information provided
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- In the guinea pig the test substance produced erythema and swelling of the earlobe. The test substance did not produce reactions in the mouse and rat ear. This indicates that Guinea-pig are more sensitive than either rat or mouse to irritant effects from the substance.
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