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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
not reported.
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.

Data source

Reference
Reference Type:
secondary source
Title:
Toxicity of Ethylene Dibromide Determined on Experimental Animals.
Author:
Rowe, V.K., Spencer, H.C., McCollister, D.D., Hollingsworth, R.L. & Adams, E.M.
Year:
1952
Bibliographic source:
Arch. Ind. Hyg. Occup. Med., Vol. 6, p.158-173.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
A 1.0% solution of ethylene dibromide in butyl carbitol acetate was applied 10 times in 14 days to the rabbit ear and bandaged onto the shaven belly.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
The material used in the skin contact test was of regular commercial quality having a purity of approximately 99%.

Test animals

Species:
rabbit
Strain:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS
Rabbits, not otherwise specified.

ENVIRONMENTAL CONDITIONS
not reported.

IN-LIFE DATES:
not reported.

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
other: butyl carbitol acetate
Controls:
not specified
Amount / concentration applied:
undiluited, 10% and 1% in butyl carbitol acetate
Duration of treatment / exposure:
10 times over 14 days
Observation period:
A period of least seven days after last exposure.
Number of animals:
Not specified
Details on study design:
Attempts to study the effects of topical application of undiluted ethylene dibromide or of strong solutions (10%) in butyl carbitol acetate by the methods usually employed in this laboratory were complicated by the finding that lethal amounts were rapidly absorbed through the intact skin.
A 1.0% solution of ethylene dibromide in butyl carbitol acetate was applied 10 times in 14 days to the rabbit ear and bandaged onto the shaven belly.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: Irritation characterisation (open exposure to the ear)
Reversibility:
fully reversible
Remarks on result:
other: When the test material was repeatedly appplied to the rabbit ear a slight irritation characterised by erythema and exfoliation was observed.
Irritation parameter:
other: Irritation characterisation (occlusive exposure to the belly)
Reversibility:
fully reversible within: 7 days
Remarks on result:
other: The repeated exposure induced a marked irritation, characterised by Erythema and edema progressing to necrosis and sloughing of the superficial layers of skin were observed. Healing was complete without scarring within seven days after the last exposure.
Irritant / corrosive response data:
On the ear it caused only a very slight irritation, characterized by erythema and exfoliation. When bandaged repeatedly onto the shaven abdomen, however, this solution caused marked irritation. Erythema and edema progressing to necrosis and sloughing of the superficial layers of skin were observed. Healing was complete without scarring within seven days after termination of exposures.
Other effects:
A few hours confinement of the material caused marked erythema and edema of the exposed areas. Where evaporation was not inhibited, slight erythema was the principal response.

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
When ethylene dibromide was applied in single or repeated doses to the rabbit ear, it caused only a slight, simple irritation. However, when confined to the skin, the material caused a severe burn and was absorbed through the skin.