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Ecotoxicological information

Long-term toxicity to fish

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Link to relevant study record(s)

Description of key information

Holcombe et al, 1985, is considered to be of adequate reliability and relevance to fulfill the regulatory endpoint for long-term toxicity testing to fish. 

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
5.81 mg/L

Additional information

Holcombe et al, 1985, was conducted in a similar manner to the EU Method C.15 (Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages) and was performed on the Japanese Killifish (Medaka) (Oryzias latipes) for an exposure period of 28 days in a flow-through freshwater test system. The test was conducted using test concentrations of 1.50, 2.52, 5.81, 9.62 & 20.3 mg/l with 120 fish exposed per concentration. Holcombe et al, 1985, reported a 28-day NOEC of 5.81 mg/l, a 28-day LOEC of 9.62 mg/l and a 28-day MATC of 7.48 mg/l based upon growth rate.

Based upon the level of reporting in the original text the study was awarded a reliability score of 2 according to the criteria of Klimisch et al, 1997, and was therefore considered to be of sufficient reliability to fulfill the regulatory endpoint.