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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

basic toxicokinetics
Type of information:
other: Paper-based toxicokinetic assessment
Adequacy of study:
key study
Study period:
The assessment was conducted in February 2013
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Summaries of studies were reviewed by a qualified toxicologist with a view to fulfilling the requirements of Annex VIII, point 8.8 of REACH.

Data source

Reference Type:
study report
Report date:

Materials and methods

Objective of study:
Test guideline
no guideline required
Principles of method if other than guideline:
In accordance with REACH Annex VIII (8.8.1) as assessment has been conducted to the extent that can be derived from the relevant available information. The assessment is based on the Guidance on information requirements and chemical safety assessment Chapter R.7c: Endpoint specific guidance.
GLP compliance:
Not relevant for assessment

Test material

Constituent 1
Chemical structure
Reference substance name:
(Z)-hex-3-enyl acetate
EC Number:
EC Name:
(Z)-hex-3-enyl acetate
Cas Number:
Molecular formula:
(Z)-hex-3-enyl acetate
Details on test material:
- Name of test material (as cited in study report): (z)-hex-3-enyl acetate (LEAC)
- Physical state: Clear colourless liquid

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on absorption:
The water solubility (1.11 g/L at 20 + 0.5°C) could allow absorption through passive diffusion. This would suggest that the gastro-intestinal tract may provide a route of absorption, following oral administration, before entering the circulatory system via the blood.

Limited absorption may also take place via the skin due to the water solubility of the test item. The substance may have skin sensitization potential and there is evidence of mild dermal irritation. Therefore damage to the skin surface may allow for increased penetration of the substance through the skin.

The low vapour pressure value (214 Pa at 25°C) shows that the substance is not available as a vapour therefore inhalation is not a significant route of exposure.
Details on distribution in tissues:
Once absorbed in the gut, the substance may be distributed in serum due to the water solubility and may therefore be distributed systemically. The water solubility would also suggest that it does not accumulate in body fat. The lack of evidence to suggest the test item is a skin sensitizer suggests that it does not bind to circulatory proteins.
Details on excretion:
There is no evidence to indicate the route of excretion but water-soluble products are not favourable for biliary excretion and therefore urinary excretion may well be a significant route for this material. Any test item that is not absorbed will be excreted in the faeces.

Metabolite characterisation studies

Metabolites identified:
Details on metabolites:
The results of the repeated dose reproductive screening study did not show evidence to indicate any test item influenced hepatic metabolism. The results of the genotoxicity asseys have shown that genotoxicity is neither enhanced nor diminished in the presence of the S9 metabolising system.

Any other information on results incl. tables

The low vapour pressure value (214 Pa at 25°C) and predicted negative explosive and oxidising properties shows that the substance is non volatile therefore inhalation is not a significant route of exposure. The substance has low water solubility (1.11 g/L). The available acute oral, acute dermal, acute inhalation and repeated dose reproductive screening studies showed limited evidence of absorption, metabolism and excretion.

The test item was non-mutagenic in bacteria or in the mouse and non-clastogenic in mammalian cells in vitro in the absence or presence of a liver enzyme metabolising system. The results of animal studies showed equivocal skin sensitisation results however in humans test results were negative. The test item is also considered a mild irritant.

Applicant's summary and conclusion

Interpretation of results (migrated information): other: See conclusion
In accordance with Annex VIII (point 8.8) of Regulation (EC) No 1907/2006 (REACH), a paper-based toxicokinetic assessment has been conducted for the substance. The available information suggests that absorption of the test substance from the gastrointestinal tract can take place. Some absorption may also take place via the skin. Once absorbed, the substance would be distributed in the serum and thereby distributed systemically and urine is the significant route of excretion. There is no evidence suggesting that the test substance may be metabolised, however no studies have been conducted to identify potential metabolites.