Nitrilotriacetonitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April-May 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well conducted and reported study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPPTS 870.1100 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Nitrilotriacetonitrile

EC Number:

230-804-9

EC Name:

Nitrilotriacetonitrile

Cas Number:

7327-60-8

Molecular formula:

C6H6N4

IUPAC Name:

2-[bis(cyanomethyl)amino]acetonitrile

Details on test material:

Batch no: 3554B
Date of receipt: 3 April 2001
Purity: 95 wt%
Moisture: 5 wt%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

The test material was mixed with corn oil; 10 mg/mL for the low dose, 40 mg/mL for the high dose.
Single gavage application at 5 mL/kg bw.

Doses:

50 and 200 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Duration of observation period following administration: 14 days
Frequency of observations and weighing: several times on the day of treatment and once daily thereafter
Necropsy of survivors performed: yes
Other examinations performed: body weight (weekly)

Statistics:

Not applicable

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 50 - < 200 mg/kg bw

Based on:

test mat.

Mortality:

None at 50 mg/kg bw
Four males and two females (60%) at 200 mg/kg bw.

Clinical signs:

other: At 50 mg/kg bw: staggered gait, wet/dark-stained urogenital area on the day of treatment At 200 mg/kg bw: hypoactivity, staggered gait, clonic convulsions, wet area around the mouth, red-stained face, lacrimation, wet urogenital area. In surviving animals

Gross pathology:

Only in animals that died (200 mg/kg bw); changes consisted of moist, red oral discharge.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Remarks:

Migrated information

Conclusions:

The oral LD50 was between 50 and 200 mg/kg bw (close to 200 mg/kg bw).

Executive summary:

An acute oral toxicity study was carried out in Sprague Dawley rats. Two groups of 5 male and 5 female rats each were dosed by single gavage at 50 or 200 mg/kg bw and kept for 14 days observation. None of the rats dosed with 50 mg/kg died; 60% mortality was observed at 200 mg/kg bw. Therefore the LD50 was between 50 and 200 mg/kg bw, close to 200 mg/kg bw.