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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April-May 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well conducted and reported study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EPA OPPTS 870.1100 (Acute Oral Toxicity)
GLP compliance:
yes
Test type:
acute toxic class method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrilotriacetonitrile
EC Number:
230-804-9
EC Name:
Nitrilotriacetonitrile
Cas Number:
7327-60-8
Molecular formula:
C6H6N4
IUPAC Name:
2-[bis(cyanomethyl)amino]acetonitrile
Details on test material:
Batch no: 3554B
Date of receipt: 3 April 2001
Purity: 95 wt%
Moisture: 5 wt%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Details on oral exposure:
The test material was mixed with corn oil; 10 mg/mL for the low dose, 40 mg/mL for the high dose.
Single gavage application at 5 mL/kg bw.
Doses:
50 and 200 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
Duration of observation period following administration: 14 days
Frequency of observations and weighing: several times on the day of treatment and once daily thereafter
Necropsy of survivors performed: yes
Other examinations performed: body weight (weekly)
Statistics:
Not applicable

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 50 - < 200 mg/kg bw
Based on:
test mat.
Mortality:
None at 50 mg/kg bw
Four males and two females (60%) at 200 mg/kg bw.
Clinical signs:
other: At 50 mg/kg bw: staggered gait, wet/dark-stained urogenital area on the day of treatment At 200 mg/kg bw: hypoactivity, staggered gait, clonic convulsions, wet area around the mouth, red-stained face, lacrimation, wet urogenital area. In surviving animals
Gross pathology:
Only in animals that died (200 mg/kg bw); changes consisted of moist, red oral discharge.

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria
Remarks:
Migrated information
Conclusions:
The oral LD50 was between 50 and 200 mg/kg bw (close to 200 mg/kg bw).
Executive summary:

An acute oral toxicity study was carried out in Sprague Dawley rats. Two groups of 5 male and 5 female rats each were dosed by single gavage at 50 or 200 mg/kg bw and kept for 14 days observation. None of the rats dosed with 50 mg/kg died; 60% mortality was observed at 200 mg/kg bw. Therefore the LD50 was between 50 and 200 mg/kg bw, close to 200 mg/kg bw.

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