tert-butyl acrylate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

wide range of treated area size

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

BASF Test: Before OECD guidelines were established, an internal standardized test was performed. 3 animals per sex per dose were treated for 24 h under occlusive conditions. Observations were performed on weekdays, weighing was performed several times during the observation period of 14 d.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

tert-butyl acrylate

EC Number:

216-768-7

EC Name:

tert-butyl acrylate

Cas Number:

1663-39-4

Molecular formula:

C7H12O2

IUPAC Name:

tert-butyl acrylate

Specific details on test material used for the study:

- Name of test material: Tertiaerbutylacrylat
- Physical state: clear liquid
- Analytical purity: ca. 99.5 %
- Impurities: stabilized with 15 ppm Hydrochinonmonomethylether

Test animals

Species:

rabbit

Strain:

Vienna White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler, D
- Mean weight at study initiation: 2.85 kg (males); 2.92 kg (females)
- Diet (e.g. ad libitum): Ssniff

ENVIRONMENTAL CONDITIONS
not reported

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: 108-247 cm2, clipped back

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.4 or 2 g/kg bw.
- Constant concentration used: yes

REMOVAL OF TEST SUBSTANCE
- Washing (if done): Lutrol/ water
- Time after start of exposure: 24 h

Duration of exposure:

24 h

Doses:

400, 2000 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7d (low dose)/ 14 d (high dose)
- Frequency of observations and weighing: observations on workdays, weighing at the beginning and the end of the test
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs- Duration of observation period following administration: 14 days (or other?)
- Frequency of observations and weighing:
- Necropsy of survivors performed: yes/no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 2 000 mg/kg bw

Mortality:

- 2000 mg/kg: 3/6 animals died
- 400 mg/kg: 0/6 animals died
see table for details

Clinical signs:

other: 2000 mg/kg: apathy, accelerated respiration, screaming

Other findings:

Local effects: obvious primary irritation effects, which were not fully reversible within the observation period; scaling at the end of the observation period.

Any other information on results incl. tables

Cumulative mortality
Dose (mg/kg bw)	sex	after 1 h	after 24 h	after 48 h	after 7 d	after 14 d
2000	male	0/3	1/3	1/3	1/3	1/3
	female	0/3	1/3	2/3	2/3	2/3
400	male	0/3	0/3	0/3	0/3
	female	0/3	0/3	0/3	0/3

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria