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Ecotoxicological information

Toxicity to soil microorganisms

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Description of key information

In a study according to OECD TG 217 with tert-butyl acrylate, the EC50 was found to be greater than the highest concentration tested.

EC50 > 1000 mg/kg soil dw (Sandy loam soil, OECD TG 217)

Key value for chemical safety assessment

Additional information

In a 28-day long-term study natural soil was exposed to tert-butyl acrylate at nominal concentrations of 0 (control), 0 (solvent control), 62.5 mg, 125 mg, 250 mg, 500 mg and 1000 mg/kg Dw in accordance with the OECD 217 guideline and in compliance with GLP criteria. On day 0, 7, 14 and 28 three aliquots were taken from the test assays. A sufficient amount of glucose was added to each aliquot and the transformation of the organic carbon of glucose to carbon dioxide was observed over measurement duration of 12 hours and expressed as the glucose induced soil respiration. The respiration rates of the replicates treated with test substance and the solvent control were compared with untreated soil samples (control). The solvent control showed no significant difference in the oxygen respiration rate. Therefore the evaluations of the inhibition effects of the test assays with test substance were carried out with the control. The 28-d EC50 was determined to be >1000 mg/kg soil Dw.

 

Supporting data from other acrylates

A supporting study, conducted according to OECD TG 217, was performed to determine the effects of a range of concentrations of methyl acrylate on the respiration of the soil micro-flora and establish the concentration which was toxic to the micro-flora. One soil type, a sandy loam soil (Lufa type 2.3), was used. The test material dissolved in water was mixed in bulk with the soil to give a range of concentrations: 63, 125, 250, 500, and 1000 mg/kg dry mass soil. The glucose-induced respiration rates in treated and control soil samples were determined at day 0, 7, and 28. The 28-d EC50 was found to be greater than the highest concentration of 1000 mg/kg dry mass (28-d EC50 > 1000 mg/kg Dw).