Registration Dossier

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23/04/1992 to 07/05 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: limited information on the soil used, not indicated whether outcome is based on soil wet weight or dry weight.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Version / remarks:
302/88 EWG
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): p-menthane
- Physical state: liquid

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Eisenia fetida
Details on test organisms:
TEST ORGANISM
- Common name: Eisenia foetida foetida
- Source: Eisenia GmbH und Co, 6200 Wiesbaden
- Age at test initiation (mean and range, SD): > 2 months
- Weight at test initiation (mean and range, SD): 300-605 mg

ACCLIMATION
- Acclimation conditions: kept on 50% peat and 50% horse or bovine manure

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d

Test conditions

Test temperature:
mean 21◦C
pH:
6.1-6.5 (6.7 at 1000 mg/kg, all worms died within 7 days)
Moisture:
40-41%
Details on test conditions:
TEST SYSTEM
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil): no data.

OTHER TEST CONDITIONS: no data

EFFECT PARAMETERS MEASURED : mortality
Nominal and measured concentrations:
nominal: 100, 180, 300, 550, 1000 mg/kg soil
Reference substance (positive control):
yes
Remarks:
chloroacetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
7 d
Dose descriptor:
LC100
Effect conc.:
1 000 other: mg/kg soil
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
300 other: mg/kg soil
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LC100
Effect conc.:
1 000 other: mg/kg soil
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
300 other: mg/kg soil
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
LC100 (7-14 d) 100 mg/kg soil
Reported statistics and error estimates:
probit

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance did not induce mortality in Eisenia foetida at concentrations of 300 mg/kg soil and below.
Executive summary:

Earthworms (Eisenia foetida) were exposed to the test substance in soil at nominal concentrations of 100, 180, 300, 550, 1000 mg/kg soil. The NOEC based on mortality was 300 mg/kg soil.