Colchicine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / micronucleus study

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

publication

Title:

Indication for thresholds of chromosome non-disjunction versus chromosome lagging induced by spindle inhibitors in vitro in human lymphocytes.Mutagenesis, 12, 133-140.

Author:

Elhajouji A, Tibaldi F and Kirsch-Volders M

Year:

1997

Bibliographic source:

Mutagenesis, 12, 133-140.

Materials and methods

Principles of method if other than guideline:

Chromosome non-disjunction was estimated on cytokinesis-blocked binucleated lymphocytes in combination with FISH using chromosome specific centromeric probes (pUC1.77 for chromosome 1 and D17Z1 for chromosome 17). This combination allows accurate evaluation of non-disjunction since artefacts are excluded from the analysis as only binucleates with the correct number of hybridization signals were taken into account. Based on mathematical models, concentrationdependent effect and number of targets necessary for induction of chromosome loss and chromosome non-disjunction were assessed.

GLP compliance:

not specified

Remarks:

No indication about GLP-compliant is reported in the paper

Type of assay:

in vitro mammalian cell micronucleus test

Test material

Test material information

Constituent 1

Reference substance name:

Colchicine

EC Number:

200-598-5

EC Name:

Colchicine

Cas Number:

64-86-8

Molecular formula:

C22H25NO6

IUPAC Name:

colchicine

Constituent 2

Reference substance name:

N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]acetamide

IUPAC Name:

N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]acetamide

Method

Species / strain

Species / strain / cell type:

lymphocytes: human

Metabolic activation:

without

Test concentrations with justification for top dose:

0.0025-0.1 mcM

Results and discussion

Test results

Species / strain:

mammalian cell line, other:

Metabolic activation:

without

Genotoxicity:

positive

Remarks on result:

other: strain/cell type: lymphocytes

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

In the absence of metabolic activation, there was a dose-related increase in the frequency of micronuclei, chromosome loss and chromosome non-disjunction.

Conc. range: 0,0025 - 0,1 microM

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive

Positive. In the absence of metabolic activation, there was a dose-related increase in the frequency of micronuclei, chromosome loss and chromosome non-disjunction.