Registration Dossier
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EC number: 294-590-9 | CAS number: 91744-28-4
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
No effects up to the limit of water solubility (WS 4-7 mg/L)
Key value for chemical safety assessment
Additional information
No experimental data investigating the acute toxicity of Glycerides, C12-18 di- and tri- (CAS No. 91744-28-4) to fish species are available. Therefore, toxicity data from a structurally related category member (Glycerides, C12-18 mono- and di- (CAS No. 91052-49-2) are used as read-across according to Regulation (EC) No. 1907/2006, Annex XI, 1.5. Both substances are esters formed from the combination of C12-18 fatty acids and glycerol. Due to differences on the degree of esterification of these substances (mono- and diester for CAS No. 91052-49-2, and di- and triester for CAS No. 91744-28-4), a higher bioavailability to aquatic organisms can be expected for the source substance (CAS No. 91052-49-2). Generally, a higher degree of esterification will result in an increase of molecular size and weight of the substance. At higher molecular size and weight, the potential to cross biological membranes tends to decrease (Guidance on information requirements and chemical safety assessment, Chapter R.11 (ECHA, 2012). Considering this information, reading across from Glycerides, C12-18 mono- and di- represents a worst-case scenario for the target substance and therefore it is justified.
One study evaluating the acute toxicity of Glycerides, C12-18 mono- and di- (CAS No. 91052-49-2) to fish species is available (Hafner, 2012). This test was conducted according to OECD 203, under GLP conditions. Danio rerio was exposed for 96 hours in a limit test to a nominal loading rate of 20 mg/L (Water Accomodated Fraction (WAF)). This concentration was chosen based on the results obtained in the tests conducted on algae and aquatic invertebrates, which resulted in EL50 values of 13 mg/L and 36.5 mg/L, respectively (loading rates). This approach is in accordance with the OECD Guidance document No. 126 “Threshold approach for acute fish toxicity” (2010).The Water Accommodated Fraction (WAF) was prepared by adding the test material into water, stirring for a period of 48 hours, followed by a sedimentation period of about 70 minutes. After the sedimentation period, the WAF was clear. Very small crumbs of test item were however, observed on the surface and at the bottom of the beakers. The test solution was prepared without a further filtration step. Analytical measurements of the test concentration were performed via TOC and DOC analysis at the start and at the end of the test. The initial concentration was determined to be 1.8 mg/L, whereas 1.9 mg/L were reported at the end of the test (DOC). The latter value corresponds to a measured test concentration of 2.7 mg/L test material.
After 96 hours of exposure to the test substance, no mortality was reported in control or treatment group and therefore, the LL50 (96 h) was determined to be > 20 mg/L (nominal, loading rate) and > 2.7 mg/L (measured final test material concentration).
Based on the data available for the structurally related category member, no acute toxicity of Glycerides, C12-18 di- and tri- (CAS No. 91744-28-4) to fish species is expected within the limit of the water solubility (WS 4-7 mg/L).
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