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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
January 12, to February 6, 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
modification in the preparation of the aqueous media
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
yes
Remarks:
modification in the preparation of the aqueous media
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Sample storage conditions before analysis: -20°C for further analysis
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dissolution
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): reconstituted water
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnid
- Strain: Daphnia magna
- Source: in-house laboratories
- Age at study initiation (mean and range, SD): less than 24 hr old
- Culture conditions: reproduction by parthenogenesis
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
21°C
pH:
7.8 - 7.9
Dissolved oxygen:
8.8- 8.9 (>98% of air saturation value)
Salinity:
Freshwater, not specified.
Nominal and measured concentrations:
Nominal: 0.10, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 & 10 mg/l
Measured test concentrations ranged from 92% to 108% of nominal.
Details on test conditions:
TEST SYSTEM
- Test vessel: polypropylene vessels
- Type (delete if not applicable): closed
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10

OTHER TEST CONDITIONS
- Photoperiod: 16 hr light/ 8 hr darkness with 20 min dawn and dusk transition periods


TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8
- Range finding study: yes
- Test concentrations: 0.10, 1.0, 10 & 100 mg/l
- Results used to determine the conditions for the definitive study: number of immobilised Daphnia
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.5 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Remarks on result:
other: 95%CL: 4.8-6.4 mg/l
Duration:
48 h
Dose descriptor:
other: EC0
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: EC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Details on results:
Results of the definitive test were inconsistent with those of the range-finding test. This has been evaluted in the report. In addition, the immobility recorded after 24h at 1.0 and 10 mg/l in the range-finding test indicated a rather flat concentration-response curve. The results of the definitive test were more convincing, showing a more clear concentration-response relationship.
Results with reference substance (positive control):
Instead of what is standard practise, no data were added on sensitivity of the daphnids by reporting the results of reference tests.
Reported statistics and error estimates:
EC50 calculation using the maximum likelihood probit analysis.
Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of diallyl phthalate to the freshwater invertebrate Daphnia magna expressed as the 48h-EC50 was 5.5 mg/l, with 95%-confidence limits of 4.8 - 6.4 mg/l and no effect at 3.2 mg/l (EC0). Measured concentrations were in agreement with nominal.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Report of testing conducted in line with the provisional procedure proposed by the Federal Environmental Agency in Germany.
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: German guideline (UBA)
GLP compliance:
not specified
Analytical monitoring:
no
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Remarks on exposure duration:
Pretest for a reproduction test
Post exposure observation period:
Not relevant
Hardness:
2.5 mmol/L expressed as total calcium carbonate
Test temperature:
Not indicated
pH:
8.0 +- 0.2
Dissolved oxygen:
Not indicated
Salinity:
Synthetic fresh water, salinity not specified.
Nominal and measured concentrations:
Not specified
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
26 mg/L
Nominal / measured:
nominal
Remarks on result:
other: Screening for reproduction test
Results with reference substance (positive control):
24h-EC50=0.35 mg/l
Reported statistics and error estimates:
Not specified.
Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of diallyl phthalate to the freshwater invertebrate Daphnia magna expressed as the 24h-EC50 was 26 mg/l with the 24h-EC0 of 11 mg/l.

Description of key information

Key information is based on a GLP study with Daphnia magna performed according to OECD guideline 202 in 2003. Analytical confirmation of test concentrations is included. Validity criteria concerning the control group and test conditions were met.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
5.5 mg/L

Additional information

The acute toxicity of diallyl phthalate to the freshwater invertebrate Daphnia magna expressed as the 48h-EC50 was 5.5 mg/l, with 95%-confidence limits of 4.8 - 6.4 mg/l and no effect at 3.2 mg/l (NOEC). Measured concentrations were in agreement with nominal. The results for the 24h EC-values are supported by those of another study (Kühn et al., 1989).