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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
January, 12 to 31, 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Samples were taken from the freshly prepared test solutions at t= 0, 24, 48 and 72 h and from the 24-h old solutions at 24, 48, 72 and 96 h.
- Sample storage conditions before analysis: -20°C
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dissolution in dechlorinated tap water
- Step one: dispersion at 500 mg/l for appr. 24 h followed by cooling to 14 ˚C and filtration (0.2 µm Gelman SuporCap filter)
- Step two: further dilution with dechlorinated tap water to prepare the test concentrations
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow Trout
- Strain: Oncorhynchus mykiss
- Source: Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK
- Length at study initiation (length definition, mean, range and SD): 4.2 cm (sd=0.2)
- Weight at study initiation (mean and range, SD): 0.86 g (sd=0.10)

ACCLIMATION
- Acclimation period: from Jan 15 to Jan 27, 2003
- Type and amount of food: commercial trout pellets
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
100 mg/l as CaCO3
Test temperature:
13.8-14.0°C
pH:
7.5-8.2
Dissolved oxygen:
9.5-10.0 mg/L (> 90% of air saturation value)
Salinity:
Fresh water, salinity not measured.
Nominal and measured concentrations:
Nominal: 0.10, 0.18, 0.32, 0.56 & 1.0 mg/l
Time-weighted mean measured: 0.094, 0.18, 0.30, 0.54 & 0.87 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass exposure vessels, covered to reduce evaporation;
- Aeration was applied using narrow bore glass tubes;
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Photoperiod: 16 hr

TEST CONCENTRATIONS
- Spacing factor for test concentrations: <2
- Range finding study: yes
- Test concentrations: 1.0, 10 & 100 mg/l (initial); 0.01 & 0.10 mg/l (second)
- Results used to determine the conditions for the definitive study: mortality in the range finding study
Reference substance (positive control):
not required
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.24 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 0.18-0.32 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.23 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 0.18-0.30 mg/L
Duration:
96 h
Dose descriptor:
other: observed LC0
Effect conc.:
0.18 mg/L
Conc. based on:
dissolved
Basis for effect:
mortality

Table: Cumulative mortality data during the definitive test

Test concentration    Mortality at different points in time (h)
Nominal Actual* 3 6 24 48 72 96
Control   0 0 0 0 0 0
0.1 0.094 0 0 0 0 0 0
0.18 0.18 0 0 0 0 0 0
0.32 0.3 0 0 0 1 10 10
0.56 0.54 0 0 4 10 10 10
1 0.87 0 0 10 10 10 10

* Chemical analysis of the old or expired test concentrations at 24, 48, 72 and 96 hours showed a marked decline of DAP in the test solution of up to 55% of the nominal value. Actual concentrations are calculated as time weighted mean measured concentrations.

Validity criteria fulfilled:
yes
Remarks:
Mortality in the control <10% at the end of the test; Constant conditions maintained; DOC > 60% of the ASV
Conclusions:
The acute toxicity of diallyl phthalate to the freshwater fish rainbow trout has been investigated and corresponded with a 96h-LC50 value of 0.24 mg/l with 95% CL of 0.18-0.32 mg/l based on nominal concentrations. The acute toxicity based on mean measured concentrations corresponded with an LC50 value of 0.23 mg/l with 95% CL of 0.18-0.30 mg/l. The highest concentration inducing no mortality or other visible abnormalities was 0.18 mg/l (LC0). These results were not significantly different to those based on nominal test concentrations.

Description of key information

K

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003. Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test conditions were met. Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003. Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test conditions were met.

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test conditions

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test condition

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test con

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and te

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test conditions were met.

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test conditions were met.

Key information is based on a GLP study with rainbow trout performed according to OECD guideline 203 in 2003.

Analytical confirmation of test concentrations is included. Validity criteria concerning mortality in the control group and test conditions were met.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.23 mg/L

Additional information

The key value for the 96h-LC50 in rainbow trout is confirmed by data from a secondary source citing a 48h-LC50 of 0.4 mg/l for another freshwater fish species (Leuciscus idus melanotus).