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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 AUG 2012 - 13 SEP 2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
other: OECD 301 D
Deviations:
no
Principles of method if other than guideline:
In biodegradation test OECD 301 D the oxygen consumption corresponded to 35.4% of the ThOD after 14 days in the toxicity control. Therefore, the test item can be considered not inhibitory to the inoculum according to this test guideline
GLP compliance:
yes
Test organisms (species):
sewage, domestic
Details on inoculum:
secondary effluent from a municipal wastewater treatment plant in Usserød, Denmark, wihich predominantly receives domestic sewage
Total exposure duration:
28 d
pH:
Start of test: 7.3 - 7.5
End of test: 7.1 - 7.5
Dissolved oxygen:
Start of test: 9.06 - 9.12
End og test: 5.99 - 6.22
Reference substance (positive control):
yes
Remarks:
Sodium benzoate
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
2.2 mg/L
Nominal / measured:
nominal
Basis for effect:
inhibition of total respiration
Key result
Duration:
28 d
Dose descriptor:
other: Oxygen consumption
Effect conc.:
35.1 other: % of ThOD
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
other: oxygen elektrode
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Validity criteria fulfilled:
yes
Conclusions:
The oxygen consumption corresponded to 35.4% of the ThOD after 14 days in the toxicity control. Therefore, the test item can be considered not inhibitory to the inoculum according to this test guideline. 
Executive summary:

Biodegradation test was done according to OECD Guideline 301D. The oxygen consumption corresponded to 35.4% of the ThOD after 14 days in the toxicity control. Therefore, the test item can be considered not inhibitory to the inoculum according to this test guideline. 

Description of key information

OECD 301D; oxygen consumption 35.4% of the ThOD 14 days ;not inhibitory to the inoculum, NOEC 2.2 mg/L

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
2.2 mg/L

Additional information

Biodegradation test was done according to OECD Guideline 301D. The oxygen consumption corresponded to 35.4% of the ThOD after 14 days in the toxicity control. Therefore, the test item can be considered not inhibitory to the inoculum according to this test guideline.