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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to terrestrial plants

Currently viewing:

Administrative data

toxicity to terrestrial plants: long-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
no data available
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable, well documented study

Data source

Reference Type:
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Disodium dihydrogen ethylenediaminetetraacetate
EC Number:
EC Name:
Disodium dihydrogen ethylenediaminetetraacetate
Cas Number:
disodium dihydrogen 2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetate
Details on test material:
no data available
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data available

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
no data available

Test substrate

not specified
Details on preparation and application of test substrate:
A silty–loamy sand agricultural soil, USDA (Soil Survey Manual), was collected from 0 to 30 cm surface layer of the Melaten field in Aachen, Germany. The soil was air-dried at room temperature, sieved through a 2-mm sieve and characterised as follows:

pH: 6.8
organic matter: 3.5 %
soil fractions
sand: 49.4 %
Clay: 42.1 %
Silt: 8.5 %

Test organisms

other: Nicotiana tabacum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Tobacco plants (N. tabacum SR-1) were used for the experiment.

Study design

Test type:
vegetative vigour test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
Total exposure duration:
21 d
approximately 3 weeks
Post exposure observation period:
no data available

Test conditions

Test temperature:
25/20 °C light/dark temperature regime
6.40 - 6.83
60% relative humidity
Details on test conditions:
16 h light period (light intensity of 320 µmol/m2 * s)
Nominal and measured concentrations:
nominal: 0.125,0.25, 1.25, 62.5, 125, and 250 mmol/kg for Na2EDTA
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
other: Nicotiana tabacum
21 d
Dose descriptor:
Effect conc.:
> 0.25 - < 1.25 other: mmol/kg
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Details on results:
EDTA did not show any adverse effects at a concentration of < 0.125 mmol/kg. At 1.25 mmol/kg of EDTA the leaves showed necrotic lesions and the dry weight was negligible. At a concentration of 0.25 mmol/kg EDTA the dry weight is only slightly lower but the standard deviation is higher in comparison to the 0.125 mmol/kg application.
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
no data availble

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Based on the results of this vegetative vigour study EDTA had an inhibitory effect on growth of Nicotiana tabacum at rate of 1.25 mmol/kg soil (equivalent to 420 mg/kg). The NOEC will be determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil).
Executive summary:

The effects of EDTA (Na2EDTA) on vegetative vigour of plants following application were examined in a laboratory test on Nicotiana tabacum. The test item was applied at a dose rate of up to 250 mmol/kg and compared to an untreated control.

According to the results, the test item had an inhibitory effect on growth of the tested species at rates of 1.25 mmol/kg soil (equivalent to 420 mg/kg) and thus determined to be the overall LOEC for this species.

The overall NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg).