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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Terpenoid biotransformation in mammals. V. Metabolism of (+)-citronellal, (±)-7-hydroxycitronellal, citral, (-)-perillaldehyde, (-)-myrtenal, cuminaldehyde, thujone, and (±)-carvone in rabbits
Author:
Ishida T., Toyota M. and Asakawa Y.
Year:
1989
Bibliographic source:
Xenobiotica, 19(8), 843-855

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
After test substance application, urine of rabbits was collected and analyzed to identify metabolites.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
not specified
Details on test material:
- Name of test material (as cited in study report): (±)-7-Hydroxycitronellal
- purchased from Nippon Terpene Chemistry Co. Ltd., and Shiono Koryo Kaisha Ltd., Japan
no further data given
Radiolabelling:
no

Test animals

Species:
rabbit
Strain:
not specified
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.5 - 3.0 kg
- Fasting period before study: 1 day
- Housing: in metabolism cages
- Diet and Water: ad libitum

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
other: Tween-80 in water (0.02 g/100 mL)
Details on exposure:
PREPARATION OF DOSING SOLUTIONS:
- samples were homogenized in water (100 ml) with Tween-80 (0.02 g) at 5°C
Duration and frequency of treatment / exposure:
once
Doses / concentrations
Remarks:
Doses / Concentrations:
about 6 g test substance per animal
No. of animals per sex per dose:
6
Control animals:
not specified
Details on dosing and sampling:
METABOLITE CHARACTERISATION STUDIES
- Tissues and body fluids sampled: urine
- Time and frequency of sampling: daily for 3 days
- From how many animals: 6
- Method type(s) for identification: silica gel column chromatography, GC-FID/TCD

Results and discussion

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
(±)-7-Hydroxycitronellol and 7-Hydroxycitronellic acid were identified in urine samples

Applicant's summary and conclusion