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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Description of key information

Key value for chemical safety assessment

Skin sensitisation

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not sensitising)
Additional information:

The skin sensitising properties of ZPS were determined in a GLP study according OECD guideline 429 (Local Lymph Node Assay (LLNA)) and EU method B.42 (Harlan CCR, 2012). For this purpose, three groups of 5 female mice each were treated with increasing concentrations of ZPS (2.5%, 5% and 10% in vehicle (w/w), respectively) applied on the dorsum of both ears (25 µL per ear) for three consecutive days. Three days after the last treatment, all animals received an intravenous injection of 3H-methyl Thymidine (3HTdR). Five hours later, the 3HTdR incorporation in the draining auricular lymph nodes was determined. The results were compared with those of a control group which was treated with the vehicle (ethanol/sterile water, 3:7 v/v). A positive control group treated with α-hexyl cinnamaldehyde (up to 25% HCA solution in acetone:olive oil, 4:1 v/v) was included in the report as a reliability check.

No signs of irritation were observed after application of a 2.5%, 5% and 10% concentration of ZPS.

Stimulation indices (SIs) of 1.14, 0.96 and 1.97 were calculated in response to a 2.5%, 5% and 10% ZPS concentration, respectively. Since the SIs were lower than 3 at all concentrations the results indicted that ZPS should not be considered a skin sensitiser (a SI of 3 is the limiting value required for classification as a skin sensitiser).

Migrated from Short description of key information:
The substance was not a skin sensitiser in a local lymph node assay in the mouse.

Justification for classification or non-classification

Based on the results of the skin sensitisation study, ZPS does not need to be classified according to Directive 67/548/EEC and according to the EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.