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Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
Details on sampling:
- The concentrations determined by spectrophotometry
Details on test solutions:
A stock solution of 3g/L (based on the active component) is prepared by disolving 16.077g of test substance in 500 mL demineralised water.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Source of supply: Hasselts aquarium
- The stock was adapted to the test conditions in LISEC lab for at least 12 days
- Toatl length: 3.2 +/-0.3 cm
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Test temperature:
20.0 - 20.9°C
7.75 - 8.26
Dissolved oxygen:
>= 7.2 mg/L
Nominal and measured concentrations:
Nominal test concentrations based on the active component: 0, 6.25, 12.5, 25, 50 and 100 mg/L
Details on test conditions:
- Renewal of the test solutions: every 24 hours
- Test containers: 3L borosilicate glass beakers
- Volume of test solution: 3 L per vessel
- Number of organisms: 10 fish for the control and for each test concentration
- Feeding: the fish are not fed during the test
- Aeration: moderate bubble-aeration for each test vessel
- Because of the photodegradation of the test substance under light, the test was carried out entirely in the dark
96 h
Dose descriptor:
Effect conc.:
50 mg/L
Nominal / measured:
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
96 h
Dose descriptor:
Effect conc.:
>= 100 mg/L
Nominal / measured:
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:

Description of key information

With high probability acutely not harmful to fish.

LC50 (96h) >= 100 mg/L (nominal) for Brachydanio rerio (OECD 203)

Key value for chemical safety assessment

Additional information

The acute aquatic toxicity on fish was investigated in a GLP guideline study according to OECD 203 for ‘zinc phthalocyanine sulfonate’ (Procter & Gamble 1994). In the study the zebrafish Danio rerio was used as test species. The fish were exposed to the substance for 96 hours in a semi-static test system. At test termination a LC50 >= 100 mg/L (nominal) was determined.