Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
8 April to 6 May 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EPA OPP 123-1 (Seedling Emergence Tier II)
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPP 123-1 (Vegetative Vigor Tier II)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): MKH 3586 70WG
- Substance type: Formulated product
- Physical state: Tan granules
- Composition of test material, percentage of components: 71.1% amicarbazone
- Batch No.: 703-0090

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Analytical dose verification was carried out for the test solutions

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
- Method of application to soil surface (if used): Spray application
- Concentration of vehicle in test medium (stock solution and final test solution): Highest rate tested = 1.5 g product in 1

Test organisms

open allclose all
Species:
Allium cepa
Plant group:
Monocotyledonae (monocots)
Species:
Beta vulgaris
Plant group:
Dicotyledonae (dicots)
Species:
Brassica napus
Plant group:
Dicotyledonae (dicots)
Species:
Daucus carota
Plant group:
Dicotyledonae (dicots)
Species:
Lactuca sativa
Plant group:
Dicotyledonae (dicots)
Species:
Lolium perenne
Plant group:
Monocotyledonae (monocots)
Species:
Lycopersicon esculentum
Plant group:
Dicotyledonae (dicots)
Species:
Zea mays
Plant group:
Monocotyledonae (monocots)
Species:
Glycine max (G. soja)
Plant group:
Dicotyledonae (dicots)
Species:
Avena sativa
Plant group:
Monocotyledonae (monocots)
Species:
other: Fagopyrum esculentum
Plant group:
Monocotyledonae (monocots)

Study design

Test type:
seedling emergence toxicity / vegetative vigour test
Study type:
extended laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
21 d

Test conditions

Details on test conditions:
TEST SYSTEM
- Testing facility: Bayer Corporation
- Test container (type, material, size): 9 cm dimameter x 8 cm tall for canola, carrot, lettuce, oat, onion, perrennial ryegrass sugar beet and tomato, 10.5 cm diameter x 12 cm tall for buckwheat, corn and soyabean.
- No. of seeds per container: Seedling emergence: for corn and soyabean, 2 seeds per container; for the other species, 5 seeds per container
- No. of plants (retained after thinning): No thinning
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Johnson County., USA
- Pesticide use history at the collection site: Not stated
- Collection procedures: Not stated
- Sampling depth (cm): Not stated
- Soil texture (if natural soil)
- % sand: 60
- % silt: 24
- % clay: 16
- Soil taxonomic classification: Sandy loam
- Soil classification system: Not stated
- Composition (if artificial substrate): NA
- Organic carbon (%): 2.5
- Maximum water holding capacity (in % dry weigth): Not stated
- CEC: 12.7

GROWTH CONDITIONS
- Photoperiod: At least 12 hours
- Light source: Natural light suplemented with sodium vapour lamps
- Light intensity and quality:
- Day/night temperatures:
- Relative humidity (%):
- Wind velocity:
- Air exchange rate:
- Watering regime and schedules:
- Water source/type:
- Volume applied:
- Interval of applications:
- Method of application:
- Any pest control method/fertilization (if used):

ACCLIMATION PERIOD:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

- Phytotoxicity rating system (if used):

VEHICLE CONTROL PERFORMED: yes/no

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Nominal and measured concentrations:
Application rates: 1, 2, 4, 8, 16, 31, 63, 125 and 250 g a.i./ha

Results and discussion

Any other information on results incl. tables

Table 1: NOEC and ER50 values for vegetative vigour and seedling emergence assessments

Species

Vegetative vigour

Seedling emergence

NOEC        (g a.i./ha)

ER50          (g a.i./ha)

NOEC        (g a.i./ha)

ER50          (g a.i./ha)

Fagopyrum esculentum(buckwheat)a

2

8.8

-

-

Brassica napus(oilseed rape)a

16

>63

31

79.5

Daucus carota(carrot)bc

-

-

31

260

Lactuca sativa(lettuce)a

4

12.2

63

131

Glycine max(soya bean)a

16

54.7

250

>250

Beta vulgaris(sugar beet)bc

2

14.6

16

74.1

Lycopersicon esculentum(tomato)a

8

29.5

63

>250

Zea mays(maize)a

63

 >250

250

>250

Avena sativa(oat)bd

63

>250

>250

>250

Alium cepa(onion)a

16

56.6

146

>250

Lolium perenne(perennial ryegrass)a

63

235

250

>250

a= endpoint based on dry weight

b= endpoint based on dry weight for vegetative vigour

c= endpoint based on survival for seedling emergence

d= endpoint based on shoot height for seedling emergence

Applicant's summary and conclusion

Conclusions:
Seedling emergence test: There were no statistically significant adverse effects on persent seedling emergence as compared to the controls for any species tested. Sugar beet was the most sensitive species tested with an ER50 value of 74.1 g a.i./ha

Vegetative vigour test: Buckwheat was the most sensitive species tested with an ER50 value of 8.8 g a.i./ha
Executive summary:

In a non-target plant study with amicarbazone, lowest ER50 values of 74.1 g a.i./ha (sugar beet) and 8.8 g a.i./ha (buckwheat) were obtained for seedling emergence and vegetative vigour, respectively.