Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 219-006-1 | CAS number: 2312-35-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26th May 1995 to 8th December 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 122-1 (Terrestrial Plant Toxicity Tier I (seedling emergence))
- Deviations:
- yes
- Remarks:
- (relative humidity/temperature/light values were outside protocol specifications on occasion, artificial soil exceeded organic content on occassion and watering rate of plants varied upon necessity determined by dryness and not a routine schedule)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of application to soil surface (if used): sprayed directly onto soil surface after the seeds were planted
- Controls: control and formulation control - Species:
- Lycopersicon esculentum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: tomato
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Cucumis sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: cucmber
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: lettuce
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: soybean
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Raphanus sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: radish
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Daucus carota
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: carrot
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: corn
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Sorghum bicolor
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: sorghum
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Lolium perenne
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: ryegrass
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Allium cepa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: onion
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Post exposure observation period:
- A minimum of 14 days after 80 % of the control plants had emerged.
- Test temperature:
- 18.9-40.2 ºC
- pH:
- 5.1-6.8
- Details on test conditions:
- TEST SYSTEM
- Testing facility: environmentally controlled plant growth room
- Test container (type, material, size): 3 x 3 " or 3.5 x 3.5 " pots
- No. of seeds per container: 1
- No. of replicates per treatment group: 30
- No. of replicates per control: 30
- No. of replicates per formulation control: 30
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 70 % sand, 20 % clay, 8 % perlite and 2 % pine bark
GROWTH CONDITIONS
- Photoperiod: 16 hours light/8 hours darkness
- Light source: metal halide lamps
- Light intensity and quality: photon fluence of 128-1835 µmol/m²/s
- Relative humidity: 33-79 %
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Day of emergence was noted and observations were made twice weekly concerning any abnormalities or phytotoxicity
- Emergence was defined as any protrusion of the plant above the soil surface; day of emergence was defined as that day when 80 % of the total number of control plants to emerge had emerged.
- Shoot length was monitored weekly and for a minimum of two weeks after plant emergence. Shoot length for all species except tomato, soybean and cucumber was measured from either the soil surface or base of the leaf rosette to the tip of the longest leaf. For tomato, soybean and cucumber, shoot length was measured from the soil surface to the apical meristem.
- Shoot dry weight was measured at the conclusion of the test; all of the plant tissue above the soil or root ball was harvested and dried to a constant weight. - Nominal and measured concentrations:
- 2.75 kg a.i./ha
- Reference substance (positive control):
- no
- Key result
- Species:
- other: Lycopersicon esculentum, Cucumis sativus, Lactuca sativa, Glycine max (G. soja), Raphanus sativus, Daucus carota, Zea mays, Sorghum bicolor, Lolium perenne and Allium cepa
- Remarks on result:
- other: Treatment at 2.75 kg a.i./ha led to decreases or increases in emergence, dry weight and/or shoot length. However, most changes were not statistically significant when compared to the controls and all were below the 25 % criterion for phytotoxic effect.
- Details on results:
- The test material had no effect on seedling emergence or growth in any of the ten species of the plant tested. Seedling emergence ranged from 83-100 %, 80-100 % and 77-100 % in the control, formulation control and treated plants, respectively. Decreases in emergence were recorded for tomato (18 %), ryegrass (17 %), radish (14 %), carrot (14 %), maize (7 %) and cucumber (4 %) following treatment with the test material while decreases in emergence were recorded for ryegrass (3 %), soybean (3 %), onion (3 %), radish (10 %), corn (10 %) and cucumber (14 %) in the formulation control groups. Onion and sorghum treated with the test material exhibited a 10 and 5 % increase in emergence compared to the controls. None of these changes in emergence were statistically significant. Day of emergence in the treated plants was not statistically significantly different to that seen in the controls.
Survival of the emergent control plants ranged from 93-100 %, while survival in the formulation controls and treated plants ranged from 92-100 and 90-100 %, respectively. These differences were not statistically different.
Following treatment with the test material, shoot length was decreased by 1, 2, 5, 8 and 5 % in lettuce, radish, carrot, soybean and sorghum, respectively, compared to controls, while dry weight of onion, radish and carrot was reduced by 13, 6 and 3 %, respectively. None of these reductions were statistically significant.
A statistically significant 23 % reduction in dry weight of sorghum was recorded although this is still below the 25 % criterion for a phytotoxic effect. Treatment with the test material stimulated plant growth in many of the species tested. There was a statistically significant increase in dry weight of tomato (26 %) and cucumber (17 %) and non-significant increases in weight of ryegrass (3 %), soybean (7 %), lettuce (10 %) and maize (3 %).
Shoot length was increased in tomato (15 %), ryegrass (2 %) and cucumber (3 %) although not significantly. Following treatment with the formulation control, shoot length was reduced in soybean (2 %), radish (7 %) and maize (10 %), dry weight was reduced in soybean (14 %), lettuce (3 %) and maize (18 %). None of these growth changes were statistically significant. Exposure to the formulation control reduced dry weight of sorghum by a statistically significant 17 % although this is still below the 25 % criterion for a phytotoxic effect. Treatment with the formulation control also stimulated growth in some species. There was a statistically significant increase in dry weight of tomato, ryegrass and carrot (22, 31 and 50 %, respectively) as well as non-significant increases in the weight of onion (5 %), radish (10 %) and cucumber (14 %). There was a significant shoot length increase in carrot and lettuce (24 and 14 %, respectively) following treatment with the formulation control as well as non-significant increases in shoot length of tomato (7 %), ryegrass (3 %) and onion (3 %). - Reported statistics and error estimates:
- The percent change in germination rate, seedling emergence and average shoot length and dry weight of treatment and formulation control seeds and plants (n2) compared to the control seeds and plants (n1) were calculated as follows:
Percent change = ((n2 - n1)/ n1) x 100
At test termination, average shoot length and dry weight of the control and formulation plants were compared using Student's t-test. If there were no significant differences between the control and formulation controls, these results were pooled and compared to the treatment plants for the appropriate measurement, shoot length or dry weight. If there were significant differences between the control and formulation controls, then the control plants were compared to the treated plants for that parameter. The Fisher's Exact test was used to determine significant differences in the emergence rates of the formulation control and treated plants. - Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the test, treatment with the test material at 2.75 kg a.i./ha led to decreases or increases in seedling emergence, dry weight and/or shoot length. However, most of these changes were not statistically significant when compared to the controls and all changes were below the 25 % criterion for phytotoxic effect. Moreover, the same decreases/increases were seen following treatment with the formulation control indicating that the active substance, propargite, in the test material is not the sole component responsible for eliciting the observed effects. It is considered that the test material will not have adverse effects on non-target terrestrial plants.
- Executive summary:
The study was conducted to evaluate the phytotoxic effect of an EC formulation containing a nominal 80 g propargite/L on the seedling emergence of ten terrestrial species in accordance with EPA OPP 122-1. Seeds of ten species were sown in plastic pots containing artificial soil of 70 % sand, 20 % clay, 8 % perlite and 2 % pine bark. Following sowing, the test material was applied at an application rate of 2.75 kg a.i./ha along with a control and formulation control. Thirty plants per species were tested in each treatment group and the pots were placed in an environmentally controlled growth room at 25 ± 2 ºC, 50 to 80 % humidity and light intensity of >250 µmol/m²/s. The day of emergence was recorded and observations of abnormalities/phytotoxicity and measurements of shoot length made. The test was continued for a minimum of 14 days after 80 % of the control plants had emerged and shoot dry weight was measured at test termination. The percent change in germination rate, seedling emergence, average shoot length and dry weight at test termination was calculated.
Under the conditions of the test, treatment with the test material at 2.75 kg a.i./ha led to decreases or increases in seedling emergence, dry weight and/or shoot length. However, most of these changes were not statistically significant when compared to the controls and all changes were below the 25 % criterion for phytotoxic effect. Moreover, the same decreases/increases were seen following treatment with the formulation control indicating that the active substance, propargite, in the test material is not the sole component responsible for eliciting the observed effects. It is considered that the test material will not have adverse effects on non-target terrestrial plants.
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26th May 1995 to 1st December 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 122-1 (Terrestrial Plant Toxicity Tier I (vegetative vigor))
- Deviations:
- yes
- Remarks:
- (relative humidity/temperature values were outside protocol specifications on occasion; a number of maize plants had 4-5 leaves instead of 3-4; min. light levels for soybean, lettuce, onion, radish, tomato and corn were <250 µmol/m2/s).
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of application to soil surface (if used): sprayed directly onto the plants with a volume sufficient to reach the maximum label rate per pot.
- Controls: control and formulation control - Species:
- Lycopersicon esculentum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: tomato
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Cucumis sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: cucmber
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: lettuce
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: soybean
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Raphanus sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: radish
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Daucus carota
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: carrot
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: corn
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Sorghum bicolor
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: sorghum
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Lolium perenne
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: ryegrass
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Species:
- Allium cepa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: onion
- Source of seed: Carolina Biological Supply, Burlington, North Carolina; Park Seed Company, Greenwood, South Carolina; W. Atlee Burpee & Cp., Warminster, Pennsylvania; Johnny's Selected Seeds, Albion, Maine - Test type:
- vegetative vigour test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Post exposure observation period:
- A minimum of 14 days after treatment with the test substance.
- Test temperature:
- 19.2-41.5 ºC
- pH:
- 6.0-6.7
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): 3 x 3 " or 3.5 x 3.5 " pots
- No. of seeds per container: 5
- No. of replicates per treatment group: 3
- No. of replicates per control: 3
- No. of replicates per vehicle control: 3
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): Metro Mix 300
GROWTH CONDITIONS
- Light source: metal halide lamps or fluorescent lighting
- Light intensity and quality: 128-1120 µmol/m²/s
- Relative humidity (%): 38-84 %
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Plant vigour observations including plant health and any abnormalities or phytotoxicity were made twice weekly.
- Weekly measurements of plant height.
- Total shoot length and dry weight were measured at the conclusion of each test. Dry weight was measured on the plant tissue above the soil or root ball after drying to a constant weight at approximately 60 ºC in a drying oven; each individual dry plant was weighed. - Nominal and measured concentrations:
- 2.75 kg a.i./ha
- Reference substance (positive control):
- no
- Key result
- Species:
- other: Lycopersicon esculentum, Cucumis sativus, Lactuca sativa, Glycine max (G. soja), Raphanus sativus, Daucus carota, Zea mays, Sorghum bicolor, Lolium perenne and Allium cepa
- Remarks on result:
- other: Treatment at 2.75 kg a.i./ha led to decreases or increases in emergence, dry weight and/or shoot length. However, most changes were not statistically significant when compared to the controls and all were below the 25 % criterion for phytotoxic effect.
- Details on results:
- The test material had no significant effects on plant vigour in any of the ten species of plant tested (all visual observations were ranked 1; 0-10 % effect compared to control). In addition, the test material had no effect on growth in any of the ten species of plant tested (all reductions in dry weight and shoot length were less than the 25 % criterion for a phytotoxic effect). Following treatment, shoot length decreased by 4% in both sorghum and radish (compared to appropriate controls), although these reductions were not statistically significant. Statistically significant shoot length reductions in lettuce (3 %), ryegrass (12 %) and cucumber were (9 %) were recorded, although these are still below the 25 % criterion for a phytotoxic effect. The only significant decrease in dry weight was for radish (16 %) but again this was below the 25 % phytotoxicity threshold. A non-significant 12 % weight reduction was recorded for sorghum. Treatment with the test material stimulated plant growth in many of the species tested. There was a statistically significant increase in shoot length of onions (9 %), soybeans (10 %) and carrots (8 %) and non-significant increases in shoot length of tomato (4 %) and corn (1 %). The test material also produced non-significant increases in the weight of soybean (12 %), lettuce (4% ), onion (9 %), ryegrass (11 %), carrot (10 %), tomato (3 %) and corn (10 %). Following treatment with the formulation control, shoot length of lettuce and sorghum were significantly reduced (8 and 6 %, respectively) while non-significant shoot length reductions were observed in radish (1 %), tomato (4 %) and soybean (3 %). There was a possible phytotoxic effect on sorghum, which had a statistically significant 31 % reduction in dry weight. A non-significant weight reduction of 12 % was recorded for onion. Treatment with the formulation control also stimulated growth in some species. There was a statistically significant increase in shoot length of onion (6 %) and corn (4 %) and non-significant shoot length increases in carrot (2 %), ryegrass (2 %) and cucumber (10 %). Dry weight of corn (10 %), lettuce (10 %), radish (4 %) , cucumber 92 %), tomato (4 %), soybean (6 %) and ryegrass (11 %) were all significantly increased compared to the control.
- Reported statistics and error estimates:
- The percent change in shoot length and dry weight of treatment plants (n2) compared to the control plants (n1) was calculated as follows:
Percent change = ((n2- n1)/ n1) x 100
At test termination, average shoot length and dry weight of the control and formulation plants were compared using Student's t-test. If there were no significant differences between the control and formulation controls, these results were pooled and compared to the treatment plants. If there were significant differences between the control and formulation controls, then the control plants were compared to the treated plants for that parameter. - Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the test, Comite had no significant effects on plant vigour in any of the ten plant species tested. Treatment with 2.75 kg/ha also led to decreases or increases in dry weight and/or shoot length. However, most of these changes were not statistically significant when compared to the controls and nearly all changes were below the 25 % criterion for a phytotoxic effect. Moreover, the same reductions were seen following treatment with the formulation blank, indicating that the active substance in the test material, propargite, is not the sole component responsible for eliciting effects. The formulation blank may be phytotoxic to sorghum (31 % reduction in weight) although this effect was not so marked on exposure to Comite (12 % reduction, non-significant and below the 25 % threshold).
- Executive summary:
The study was conducted to evaluate the phytotoxic effect of an EC formulation containing a nominal 80 g propargite/L on the vegetative vigour of ten terrestrial species in accordance with EPA OPP 122-1. Seeds of ten species were sown in plastic pots containing artificial soil (Metro Mix 300). Following sowing all plants were grown for one week post-emergence to the 3-4 leaf stage (4-5 leaf stage for corn), the test material was applied to an application rate of 2.75 kg a.i./ha along with a control and formulation control. Fifteen plants per species were tested in each treatment group and the pots were placed in an environmentally controlled growth room at 25 ± 2 ºC, 50 to 80 % humidity and light intensity of 100-300 µmol/m²/s. Plant vigour observations were made twice weekly, plant height was recorded weekly while shoot length and dry weight were recorded at test termination. The test was continued for a minimum of 14 days after the treatment of plants. The percent change in shoot length and dry weight at test termination was calculated.
Under the conditions of the test, treatment with the test material at 2.75 kg a.i./ha had no significant effect on plant vigour in any of the plant species tested. Treatment also led to decreases or increases in dry weight and/or shoot length. However, most of these changes were not statistically significant when compared to the controls and nearly all changes were below the 25 % criterion for a phytotoxic effect. Moreover, the same reductions/increases were seen following treatment with the formulation control, indicating that the active substance, propargite, in the test material is not the sole component responsible for eliciting the observed effects. It is considered that the test material will not have adverse effects on non-target terrestrial plants.
Referenceopen allclose all
Table 1: Seedling emergence
Species | Treatment | Emergence day | Max. no. emerged | % emerged | % change | Final no. surviving |
Tomato | Control | 8 | 29 | 97 | - | 29 |
Formulation control | 8 | 29 | 97 | 0 | 28 | |
Test material | 7 | 24 | 80 | -18 | 24 | |
Ryegrass | Control | 5 | 30 | 100 | - | 30 |
Formulation control | 5 | 29 | 97 | -3 | 29 | |
Test material | 6 | 25 | 83 | -17 | 25 | |
Soybean | Control | 9 | 27 | 90 | - | 25 |
Formulation control | 9 | 26 | 87 | -3 | 26 | |
Test material | 11 | 27 | 90 | 0 | 27 | |
Lettuce | Control | 3 | 28 | 93 | - | 27 |
Formulation control | 3 | 30 | 100 | +7 | 30 | |
Test material | 3 | 28 | 93 | 0 | 28 | |
Onion | Control | 8 | 27 | 90 | - | 26 |
Formulation control | 8 | 26 | 87 | -3 | 24 | |
Test material | 7 | 30 | 100 | +10 | 27 | |
Radish | Control | 5 | 27 | 90 | - | 27 |
Formulation control | 4 | 24 | 80 | -10 | 24 | |
Test material | 5 | 23 | 77 | -14 | 21 | |
Carrot | Control | 9 | 27 | 90 | - | 27 |
Formulation control | 9 | 29 | 97 | +8 | 29 | |
Test material | 8 | 23 | 77 | -14 | 23 | |
Corn | Control | 6 | 29 | 97 | - | 28 |
Formulation control | 7 | 26 | 87 | -10 | 26 | |
Test material | 5 | 27 | 90 | -7 | 27 | |
Cucumber | Control | 4 | 29 | 97 | - | 29 |
Formulation control | 4 | 25 | 83 | -14 | 25 | |
Test material | 4 | 28 | 93 | -4 | 28 | |
Sorghum | Control | 5 | 25 | 83 | - | 25 |
Formulation control | 5 | 26 | 87 | +5 | 25 | |
Test material | 5 | 26 | 87 | +5 | 26 |
Table 2: Growth
Species | Treatment | Ave. shoot length (cm) | Percent change | Ave. shoot dry weight (g) | Percent change |
Tomato | Control | 11.4 | - | 0.584 | - |
Formulation control | 12.2 | +7 | 0.712 | +22 | |
Test material | 13.6 | +15 | 0.740 | +26 | |
Ryegrass | Control | 33.1 | - | 0.197 | - |
Formulation control | 34.2 | +3 | 0.259 | +31 | |
Test material | 34.3 | +2 | 0.202 | +3 | |
Soybean | Control | 30.5 | - | 2.014 | - |
Formulation control | 29.8 | -2 | 1.741 | -14 | |
Test material | 27.6 | -8 | 2.007 | +7 | |
Lettuce | Control | 10.8 | - | 0.501 | - |
Formulation control | 12.3 | +14 | 0.488 | -3 | |
Test material | 10.7 | -1 | 0.542 | +10 | |
Onion | Control | 14.4 | - | 0.022 | - |
Formulation control | 14.9 | +3 | 0.023 | +5 | |
Test material | 14.7 | 0 | 0.020 | -13 | |
Radish | Control | 17.8 | - | 0.671 | - |
Formulation control | 16.5 | -7 | 0.735 | +10 | |
Test material | 16.9 | -2 | 0.661 | -6 | |
Carrot | Control | 7.6 | - | 0.034 | - |
Formulation control | 9.4 | +24 | 0.051 | +50 | |
Test material | 7.2 | -5 | 0.033 | -3 | |
Corn | Control | 57.3 | - | 0.652 | - |
Formulation control | 51.5 | -10 | 0.535 | -18 | |
Test material | 54.5 | 0 | 0.616 | +3 | |
Cucumber | Control | 7.5 | - | 0.724 | - |
Formulation control | 7.5 | 0 | 0.825 | +14 | |
Test material | 7.7 | +3 | 0.900 | +17 | |
Sorghum | Control | 20.6 | - | 0.092 | - |
Formulation control | 20.6 | 0 | 0.076 | -17 | |
Test material | 19.6 | -5 | 0.071 | -23 |
Table 1: Growth values
Species | Treatment | Ave. shoot length (cm) | Percent change | Ave. shoot dry wt (g) | Percent change |
Soybean | Control | 39.6 | - | 2.751 | - |
Formulation control | 38.6 | -3 | 2.904 | +6 | |
Test material | 43.1 | +10 | 3.171 | +12 | |
Lettuce | Control | 14.4 | - | 1.192 | - |
Formulation control | 13.3 | -8 | 1.306 | +10 | |
Test material | 14.0 | -3 | 1.295 | +4 | |
Sorghum | Control | 60.1 | - | 1.130 | - |
Formulation control | 56.4 | -6 | 0.784 | -31 | |
Test material | 57.8 | -4 | 0.991 | -12 | |
Onion | Control | 30.0 | - | 0.212 | - |
Formulation control | 31.8 | +6 | 0.187 | -12 | |
Test material | 32.6 | +9 | 0.216 | +9 | |
Radish | Control | 21.3 | - | 0.931 | - |
Formulation control | 21.0 | -1 | 0.968 | +4 | |
Test material | 20.2 | -4 | 0.799 | -16 | |
Ryegrass | Control | 52.0 | - | 0.693 | - |
Formulation control | 52.8 | +2 | 0.701 | +1 | |
Test material | 46.3 | -12 | 0.770 | +11 | |
Tomato | Control | 31.0 | + | 3.178 | + |
Formulation control | 29.9 | -4 | 3.317 | +4 | |
Test material | 31.5 | +4 | 3.353 | +3 | |
Carrot | Control | 20.9 | - | 0.660 | - |
Formulation control | 21.4 | +2 | 0.662 | 0 | |
Test material | 22.8 | +8 | 0.730 | +10 | |
Corn | Control | 113.4 | - | 3.932 | - |
Formulation control | 118.3 | +4 | 4.319 | +10 | |
Test material | 114.7 | +1 | 4.550 | +10 | |
Cucumber | Control | 60.8 | - | 6.263 | - |
Formulation control | 66.7 | +10 | 6.381 | +2 | |
Test material | 57.9 | -9 | 6.296 | 0 |
Description of key information
Changes to seedling emergence and plant vigour were all below the 25 % criterion for phytotoxic effect; studies conducted in accordance with EPA OPPS 122-1; Aufderheide and Kranzfelder, 1995a/b
Key value for chemical safety assessment
Additional information
In a GLP compliant seedling emergence test conducted in line with EPA OPP 122-1, the effect of the test material on seedling emergence was determined in ten species at an application rate of 2.75 kg/ha. Under the conditions of the test, decreases or increases in seedling emergence, dry weight and/or shoot length were observed. However, most of these changes were not statistically significant when compared to the controls and all changes were below the 25 % criterion for a phytotoxic effect. Similar decreases/increases were seen with the formulation control indicating that propargite is not the sole component responsible for eliciting the observed effects.
In a GLP compliant vegetative vigour test conducted in line with EPA OPP 122-1, the effect of the test material on vegetative vigour was determined in ten species at an application rate of 2.75 kg/ha. Under the conditions of the test, no significant effect was seen in plant vigour. Decreases or increases in dry weight and/or shoot length were seen although most of these changes were not statistically significant when compared to the controls and nearly all changes were below the 25 % criterion for a phytotoxic effect. Similar decreases/increases were seen with the formulation control indicating that propargite is not the sole component responsible for eliciting the observed effects.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
