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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
other company data
Title:
Unnamed
Year:
1966
Report date:
1966
Reference Type:
secondary source
Title:
Unnamed
Year:
2000
Reference Type:
secondary source
Title:
SIDS Initial Assessment Report - 2-Methylbut-3-yn-ol
Author:
BASF AG
Year:
2002
Bibliographic source:
OECD SIDS

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Internal BASF method was used
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylbut-3-yn-2-ol
EC Number:
204-070-5
EC Name:
2-methylbut-3-yn-2-ol
Cas Number:
115-19-5
Molecular formula:
C5H8O
IUPAC Name:
2-methylbut-3-yn-2-ol
Details on test material:
- Analytical purity: 98.5%

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 3.00, 2.54 kg

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated other eye of same animal
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 ml
- Concentration (if solution): 100%
Duration of treatment / exposure:
8 d
Observation period (in vivo):
8 d
Number of animals or in vitro replicates:
2
Details on study design:
SCORING SYSTEM: Findings were scored according internal BASF gradings, which could be converted to scoring acc. Draize

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
2.16
Max. score:
4
Reversibility:
not reversible
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
1
Max. score:
4
Reversibility:
not reversible
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
2
Max. score:
3
Reversibility:
not reversible

Any other information on results incl. tables

Irritation scores acc. Draize:

Readings Animal Conjunctiva Chemosis Corneal opacity Additional findings
1h 1 2 3 2  
2 2 3 2  
3 h 1 2 3 2 dermal ablation
2 2 3 2 dermal ablation
24 h 1 2 1 2 dermal ablation
2 2 1 2 bd
48 h 1 2 1 2  
2 2 1 1 bd
72 h 1 2 1 3  
2 2 1 3  
6 d 1 2 nd 3 ex
2 2 1 3  
8 d 1 2     st, cv
2 1   1  
Mean 24 - 72 h 1 2 1 2.3  
2 2 1 2  
Mean   2 1 2.16

bd: bloody discharge

cv: corneal vascularisation

st: staphyloma

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye)
Remarks:
Migrated information Criteria used for interpretation of results: EU