Stearoyl chloride

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets scientific standards with acceptable restrictions (partly limited documentation, e.g. no details about the test substance)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

no guideline available

Guideline:

other:

Deviations:

no

Principles of method if other than guideline:

Young adult laboratory mice were purchased from a breeder. Usually the source and strain of the animals were not documented. Several groups of 5 mice per sex and dose were treated simultaneously by ip injections with preparations of the test substance in a suitable vehicle.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Stearic acid chloride

IUPAC Name:

Stearic acid chloride

Details on test material:

- Name of test material (as cited in study report): Stearinsäurechlorid
- Physical state: liquid
- Analytical purity: 98 %
- pH: acidic
- melting point: 7,3

Test animals

Species:

mouse

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 31-42 g (male), 24-35 g (female)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: water with adjuvant

Doses:

200, 250, 320, 400, 800, 1600 ml/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
clinical observation:10 min; 4h, 6h p.a. on day of administration, once daily the following days exept on weekend and holidays
weighing: only at the beginning of the study for dose calculation
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 203 mg/kg bw

Remarks on result:

other: calculated using a density of 0.9 g/ml for stearic acid chloride

Mortality:

1600 ml/kg: 5/5 (male) 5/5 (female)
800 ml/kg: 5/5 (male) 5/5 (female)
400 ml/kg: 5/5 (male) 5/5 (female)
320 ml/kg: 5/5 (male) 5/5 (female)
250 ml/kg: 5/5 (male) 4/5 (female)
200 ml/kg: 1/5 (male) 0/5 (female)
death occured in a time period fom 24 h p.a. until 10 day p.a.

Clinical signs:

Tremor, twitching, convulsions accelerated discontinuous breathing, high steepy gait directly after injection.
closed eyes, discontinuous breathing, abdominal postition until day 9 after administration. Surviving animals without syptoms fom day 10 onward.

Body weight:

only determined at the beginning of the study for applying the right dose: 31-42 g (male), 24-35 g (female)

Gross pathology:

peritional adhesive desease

Applicant's summary and conclusion

Executive summary:

Several groups of 5 mice per sex and dose were treated simultaneously by ip injections with preparations of the test substance in a suitable vehicle. The LD50 after ip application is about 225 ml/kg in mice. based on the tensity of 0.9 g/ml this calculates to a LD 50 of 203 mg/kg.

Conclusion

The LD50 after ip application is about 225 ml/kg in mice. based on the tensity of 0.9 g/ml this calculates to a LD 50 of 203 mg/kg.