N-methylaniline

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: 2e. Study well documented, meets generally accepted scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Guideline:

other: NEN 6501 (1980) and OECD 201 (older version)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Aniline

EC Number:

200-539-3

EC Name:

Aniline

Cas Number:

62-53-3

Molecular formula:

C6H7N

IUPAC Name:

aniline

Constituent 2

Reference substance name:

2-methylaniline

IUPAC Name:

2-methylaniline

Constituent 3

Reference substance name:

3-methylaniline

IUPAC Name:

3-methylaniline

Constituent 4

Reference substance name:

4-methylaniline

IUPAC Name:

4-methylaniline

Details on test material:

Aniline: purity = pro analysis; LogPoct=1.03 (0.90); Mol weight= 93.13;Chs= 0.00
2-methylaniline: purity >= 99.5%; LogPoct=1.54; Mol weight= 107.16;Chs= 0.10
3-methylaniline: purity >= 99%; LogPoct=1.54; Mol weight= 107.16;Chs= - 0.07
4-methylaniline: purity > 97%; LogPoct=1.54; Mol weight= 107.16;Chs= -0.17
Chs: Hammett sigma constant, which is dependent on the position of the substituent.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): isopropanol

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Age at study initiation (mean and range, SD): < 24 hr

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.08 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Aniline: 95% C.I.:0.06-0.10 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.52 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 2-Methylaniline: 95% C.I.: 0.36-0.81 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 3-Methylaniline

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 4-methylaniline: 95% C.I.:0.14-0.24 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

All anilines tested were acutely toxic to D. magna at concentrations below 1 mg/L. Compared to the methylated anilines, aniline was the most toxic.