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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study conducted by GLP accredited lab according to OECD guideline method 474

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
GLP compliance:
yes (incl. QA statement)
Type of assay:
micronucleus assay

Test material

Constituent 1
Reference substance name:
Tin(II) bis(methanesulfonate)
EC Number:
401-640-7
EC Name:
Tin(II) bis(methanesulfonate)
IUPAC Name:
tin(II) bis(methanesulfonate)
Constituent 2
Reference substance name:
53408-94-9
EC Number:
610-996-4
Cas Number:
53408-94-9
IUPAC Name:
53408-94-9

Test animals

Species:
mouse
Strain:
CF-1
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
distilled water
Details on exposure:
after dose finding study the maximum tolerated dose was determined to be 1000mg/kg
Duration of treatment / exposure:
72h
Frequency of treatment:
one single treatment at the beginng of the study
Post exposure period:
mice were killed at 24,48 and 72h after exposure
Doses / concentrations
Remarks:
Doses / Concentrations:
1000mg/kg
Basis:
actual ingested
No. of animals per sex per dose:
15
Control animals:
yes, concurrent vehicle
Positive control(s):
5 male and 5 female mice , 50mg/kg of cyclophosphamide

Examinations

Tissues and cell types examined:
bone marrow from femur
Details of tissue and slide preparation:
femur dissected and aspirated with foetal calf serum and bone marrow smears prepared following centrifugation and re-suspention. The smears were air-dried, fixed in absolute methanol and stained in May-Grunwald/Giemsa
Evaluation criteria:
stained bone marrow smears were examinated at random using light microscopy at 1000x magnification.

Results and discussion

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative