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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The reliability rating is a 2 because the study procedure was equivalent to an OECD standard guideline, which describes a procedure designed to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was not conducted under GLP.

Data source

Reference
Reference Type:
other: Study report, company data
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Substance type: commercial product

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The organisms used in this study were obtained from laboratory stocks cultured at EG&G Bionomics. Culture organisms were fed a combination of Rnagen (R) Salmon Starter and unicellular green algae.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
Ranged from 140 to 180 mg/L as CaCO3
Test temperature:
Ranged from 21 to 23 degrees C
pH:
Ranged from 7.7 to 8.5
Dissolved oxygen:
Ranged from 7.7 to 8.4 mg/L
Salinity:
freshwater
Nominal and measured concentrations:
Nominal concentrations were: 0.92, 1.6, 2.7, 4.5, and 7.6 mg/L
Details on test conditions:
Each test solution was prepared by adding an aliquot of test material to 1 L of dilution water and mixing on a magnetic stir plate for 4 hours. The test chambers were 150 beakers. Each treatment level was tested in triplicate. Fifteen water fleas (5 per beaker), less than or equal to 24 hours old were tested per treatment level. Observations and water quality samples were taken at time 0 and every 24 hours. Test beakers were sealed to prevent volatile loss of test substance. The test area was illuminated with Durotest fluorescent lights at an intensity of 100 to 150 footcandles. Test solutions were not aerated.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
6.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 5.3 to 7.6 mg/L (95% CL)
Details on results:
Percent mortality was reported as:

Loading Level 48 Hour Mortality
(mg/L) (%)
Control 0
0.92 7
1.6 33
2.7 27
4.5 13
7.6 73

Several of the surviving water fleas were lethargic in the 7.6, 4.5, and 1.6 mg/L treatment levels.
Reported statistics and error estimates:
Effect concentrations were estimated by the moving average angle method.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of Alcohols, C9 -11 -iso, C10 -rich, as measured by immobility to the water flea (Daphnia magna) was evaluated in freshwater. The 48-h EL50 for Daphnia magna was 6.2 mg/l, based on nominal loading of the test substance in water.
Executive summary:

The acute toxicity of Alcohols, C9 -11 -iso, C10 -rich, as measured by immobility to the water flea (Daphnia magna) was evaluated in freshwater. The 48-h EL50 for Daphnia magna was 6.2 mg/l, based on nominal loading of the test substance in water.