Registration Dossier

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP screening study
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report date:
1998

Materials and methods

Principles of method if other than guideline:
Ames tests in two bacterial strains (S. typhimurium TA98 and TA100).
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-dichloro-5-isopropoxyaniline
EC Number:
255-258-9
EC Name:
2,4-dichloro-5-isopropoxyaniline
Cas Number:
41200-96-8
Molecular formula:
C9H11Cl2NO
IUPAC Name:
2,4-dichloro-5-isopropoxyaniline
Details on test material:
- Name of test material (as cited in study report): 2,4-dichloro 5-isopropoxy aniline (DIA)
- Storage condition of test material: stored in the dark at room temperature in air-tight containers

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA98 and TA100
Metabolic activation:
with and without

Results and discussion

Any other information on results incl. tables

No indication of the test results provided

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive with metabolic activation
negative without metabolic activation

Under the test conditions, 2,4-Dichloro-5-isopropoxyaniline induced a strong mutagenic activity in the bacterial strains S. typhimurium TA98 and TA100 with metabolic activation only.
Executive summary:

In a reverse gene mutation assay in bacteria performed in compliance with the GLP, Salmonella typhimurium TA98 and TA100 were exposed to 2,4-Dichloro-5-isopropoxyaniline (named as 2,4-dichloro 5-isopropoxy aniline (DIA) in the report). A strong mutagenic activity in both strains was observed with metabolic activation only.