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Short-term toxicity to fish

In a static mortality test, young Zebrafish were exposed to the test item for 96 hours according to OECD 203 (1992). The 96 -hour LC50 was determined to be > 100 mg/L and the 96 -hour NOEC to be 100 mg/L.

Short-term toxicity to aquatic invertebrates

In a static immobilization test, young Daphnia magna (< 24 h old) were exposed to the test item for 48 hours according to OECD 202 (2004). The 48 h EC50 was determined to be 8.3 mg/L and the NOEC was determined to be 1.6 mg/L (all calculations were based on the nominal concentrations).

 

Toxicity to aquatic algae and cyanobacteria

In a static growth inhibition test, exponentially-growing cultures of unicellular green algal species Pseudokirchneriella subcapitata (formerly known as Selenastrum capricornutum) were exposed to the test item for 72 hours according to OECD 201 (2011). The 72-h EC50 based on the growth rate was determined to be 11.1 mg/L, the 72-h EC10 based on the growth inhibition was determined to be 1.8 mg/L and the NOEC was determined to be < 0.5 mg/L (all calculations were based on the nominal concentrations).

Toxicity to microorganisms

The influence of the test item on the activity of the activated sludge was estimated after 3 -hour test performed according to OECD 209 (2010). The EC50 value of test item was determined as 732.6 mg/L (95% confidence limits: 517.8 – 1036.6 mg/L). Based on the statistical and biological evaluation in this test the NOEC was determined as 25.6 mg/L.