Fatty acids, tall-oil, reaction products with bisphenol A, epichlorohydrin, glycidyl tolyl ether and triethylenetetramine

Endpoint summary

Description of key information

A study was performed to determine the effects of TOFA_TETA_PAA_BADGE_CGE_Adduct on the reproduction and growth of the earthworm, Eisenia fetida, in an artificial soil under laboratory conditions. The method followed was that described in OECD 222 Guideline for the testing of Chemicals, Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei) 2004. The NOEC for mortaliity, parental body weight and number of juveniles was determined to by 1000 mg/kg bw/d

A terrestrial plant study (OECD 208) was performed to determine the effects of TOFA_TETA_PAA_BADGE_CGE_Adduct. There were no treatment-related effects on plant germination or survival for carrot, lettuce, tomato, oat or onion for all species at rates of up to 1000 mg/kg dry soil. There was a 16.7% reduction in germination for cabbage at 333.33 mg/kg dry soil and a 5.1% reduction in survival at 1000 mg/kg dry soil. Based on visual observations, there were no effects of >10% at rates up to 1000 mg/kg dry soil for any of the species tested. The EC25 values for height were estimated to be >1000 mg/kg dry soil for cabbage, lettuce, tomato, oat and onion and 387.92 mg/kg dry soil for carrot. The EC50 values for height were estimated to be >1000 mg/kg dry soil for all species. The NOEC values for effects on height were 1000 mg/kg dry soil for cabbage, lettuce, oat and onion, 111.11 mg/kg dry soil for carrot and 333.33 mg/kg dry soil for tomato. The EC25 values for dry weight were estimated to be 800.42 and 548.29 mg/kg dry soil for cabbage and carrot respectively and >1000 mg/kg dry soil for lettuce, tomato, oat and onion. The EC50 values for dry weight were estimated to be 878.35 mg/kg dry soil for carrot and >1000 mg/kg dry soil for all other species. The NOEC values for effects on dry weight were 333.33 mg/kg dry soil for cabbage and carrot and 1000 mg/kg dry soil for lettuce, tomato, oat and onion. The study was considered valid as the validation criteria were met and no visible treatment related effects were observed in the controls. Due to effects on seedling height the carrot was considered to be the most sensitive species.

An OECD 216 soil microorganisms: Nitrogen Transformation Study was performed to assess the long-term effect of the test item, after a single exposure, on the nitrogen transformation activity of soil microorganisms.

Soil microorganisms were exposed to the test item at concentrations of 10, 32, 100, 320 and 1000 mg/kg for 28 days at temperatures of between 19 and 21°C in the dark with the addition of powdered Lucerne-green-grass meal to act as a respiratory substrate.

The inhibitory effect of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0 and 28 and compared to data obtained from solvent control soil samples.

The test item showed no significant effect on the nitrogen transformation activity of soil microorganisms at the test concentrations of 10, 32, 100, 320 and 1000 mg/kg over a 28-Day period and therefore can be considered to have no long-term effect on nitrogen transformation in soil.

For risk assessment purposes the NOEC value of 111.11 mg/kg dry soil based on the most sensitive endpoint, seedling height for carot and therefore this will be used to derive soil PNEC values