Zinc O,O,O',O'-tetrabutyl bis(phosphorodithioate)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-guideline study; use of non-standard vehicle for dosing

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phosphorodithioic acid, mixed O,O-bis(1,3-dimethylbutyl and iso-Pr) esters, zinc salts

EC Number:

283-392-8

EC Name:

Phosphorodithioic acid, mixed O,O-bis(1,3-dimethylbutyl and iso-Pr) esters, zinc salts

Cas Number:

84605-29-8

IUPAC Name:

zinc bis[O-(1,3-dimethylbutyl) O-isopropyl dithiophosphate]

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 6-7 weeks
- Weight at study initiation: 160 to 200 g (male), 140-180 g (female)
- Housing: Individually in stainless steel cages
- Diet: ad libitum
- Water: ad libitum


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3
- Humidity (%): 30-70
- Air changes (per hr): 10

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: 10% aqueous dispersion of gum Arabic

Details on dermal exposure:

TEST SITE
- Area of exposure: Dorsal zone
- Type of wrap if used: aluminum foil fastened by a bandaid


REMOVAL OF TEST SUBSTANCE
- Washing: rinsed with warm water
- Time after start of exposure: 25 hours


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 4 mL/kg
- Concentration (if solution): 50.04 g/100mL
- Constant volume or concentration used: yes


VEHICLE
- Amount(s) applied (volume or weight with unit): 4 mL/kg

Duration of exposure:

25 hours

Doses:

0 and 2002 mg/kg

No. of animals per sex per dose:

5 males and 5 females

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 1, 7, and 14 days
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight,organ weights

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 002 mg/kg bw

Mortality:

0 mg/kg: 0/10 dead
2002 mg/kg: 0/10 dead

Clinical signs:

other: Prostration in one animal. No other behavioural anomalies. Desquamation of the skin noted on test day 11.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Based on the low level of toxicity and no mortality observed at 2002 mg/kg, the test material is not classified for acute dermal toxicity.
CLP: not classified
DSD: not classified