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Sika Hardener LG was assessed in two aquatic toxicity studies: acute toxicity to aquatic invertebrates (Daphnia) and acute toxicity to aquatic algae.

In the short term toxicity to aquatic invertebrates study according to EU-method C.2 and OECD guideline 202. Young Daphnia were exposed in a semi-static test to test item at nominal concentrations of up to 100 mg/L. The 48h-NOEC (EC0) was determined to be 50.0 mg/L, the 48h-EC50 was calculated to be 125.5 mg/mL and the 48h-EC100 was determined to be >100.0 mg/mL.

The effect of the test item Sika Hardener LG on the growth of an unicellular green algal species Pseudokirchneriella subcapitata (formerly: Selenastrum capricornutum) was assessed according to EU-method C.3 and OECD guideline 201. Exponentially growing cultures of Pseudokirchneriella subcapitata were exposed to various concentrations of the test item (6.25; 12.5; 25.0; 50.0 and 100.0 mg/L) over several generations under defined conditions. The shape of the algal cells growing was obviously not affected. The test item had a statistically significant inhibitory effect on the growth based on the average specific growth rate, yield and areas under the growth curves of Pseudokirchneriella subcapitata after the 72 exposure period in the concentration range tested. A test concentration of 50.0 mg/mL of the was determined as the 72-hour LOEC. A test concentration of 25.0 mg/mL was determined as the 72-hour NOEC. The 72- hour EC50 based on growth rate was determined as 51.9 mg/mL, a EC50 based on yield of 38.5 mg/mL.