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Fish:

The acute toxic effects of EDDHMA-Fe to fish were investigated in studies according to the principles of OECD-Guideline 203 and EU method C.1. The LC50 for a 96 hour exposure was greater than 1000 mg/L for rainbow trout and carp.

 

Daphnia:

The acute toxic effects of EDDHMA-Fe to Daphnia magna were investigated according to the principles of OECD-Guideline 202 and EU method C.2. The EC50 for a 48 hour exposure was 4.6 g/L.

In a long-term study, EDDHMA-Fe had no effect on the survival of Daphnia magna at concentrations up to and including 1000 mg/L. A NOEC of 320 mg/L was derived based on reproduction and greater than or equal to 1000 mg/L based on parental mortality.

 

Algae:

The 72 hour ErC50 value for EDDHMA-Fe with the Selenastrum capricornutum was 71 mg/L and the ErC10 was 2.8 mg/L.

However, due to the chelating properties and coloration of the test solutions, results on algae should be considered with care. The end-point for aquatic plants might be more representative.

 

Aquatic plants:

In a guideline study on the acute toxicity of EDDHMA-Fe to Lemna minor the EC50-values for inhibition of log biomass growth (EbC50), specific growth rate (ErC50) and log biomass dry weight (EdwC50) were all > 184.2 mg/L, the NOEC was 184.2 mg/L and the LOEC was > 184.2 mg/L.

 

Microorganisms:

The 3 hours EC10 value for the (read across) test substance EDDHA-FeNa in the Activated Sludge Respiration Inhibition Test was 450 mg/L. The EC50 was greater than 1000 mg/L. Recalculated based on molecular mass correction this would give for EDDHMA-Fe 477 mg/L and > 1060 mg/L for EC10 and EC50 value respectively.