3-diethylaminophenol

Repeated dose toxicity: oral

Administrative data

Endpoint:

short-term repeated dose toxicity: oral

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no guideline study, limited documentation, limited number of endpoints assessed

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1957

Report date:

1957

Materials and methods

Principles of method if other than guideline:

Method: BASF-Test used before OECD Test Guidelines were in place

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-diethylaminophenol

EC Number:

202-090-9

EC Name:

3-diethylaminophenol

Cas Number:

91-68-9

Molecular formula:

C10H15NO

IUPAC Name:

3-(diethylamino)phenol

Details on test material:

- Name of test material (as cited in study report): Diäthyl-m-aminophenol
- Physical state: solid
- Analytical purity: 99 %
- Other: soluble in acetic acid, alcohol, lutrol, ethylenechloride, unsoluble in soda solution and vegetable oil

Test animals

Species:

cat

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2 - 4 kg

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Traganth

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 1 %, 2 % or 10 %

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

- 2,4, 12 applications
- 2 cats received 2 applications with 100 or 1000 mg/kg bw ; 1 cat received 4 applications with 50 mg/kg bw ; 1 cat received 12 applications with 50 mg/kg bw

Frequency of treatment:

- daily

Doses / concentrations

Remarks:

Doses / Concentrations:
50; 100 or 1000 mg/kg bw
Basis:
actual ingested

No. of animals per sex per dose:

1 or 2 cats

Control animals:

no

Positive control:

no

Examinations

Observations and examinations performed and frequency:

CAGE SIDE OBSERVATIONS: Yes

BODY WEIGHT: Yes

HAEMATOLOGY: Yes, including methemoglobin

URINALYSIS: Yes

Sacrifice and pathology:

GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: No

Results and discussion

Results of examinations

Details on results:

Animals which received the higher doses (100 or 1000 mg/kg bw) died after the second application. Symptoms like excessive salivation, vomiting after application as well as ataxia and somnolence were observed. The methemoglobin tests were positive. In both animals the lungs and the liver were dark coloured, the heart was reddishliy dicoloured and/or showed sporadic bleedings. Also the spleen was dark colored and enlarged.

One of the cats which received 50 mg/kg bw died 2 days after the fourth application. Vomiting and salivation were observed. The methaemoglobin tests were positive and the number of haemoglobin decreased constantly. Liquids were detected in the lungs and the intraperitoneally adipose tissue was yellow-coloured.

One animal survived 12 applications of 50 mg/kg bw, only after the first application salivation and vomiting was noticed. No more clinical signs or symptoms were observed after the other applications. No loss of body weight was detected. During the exposure time the number of haemoglobin and of the erythrocytes decreased (haemolytic anaemia).

Target system / organ toxicity

Critical effects observed:

not specified