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Long-term toxicity to aquatic invertebrates

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No standard toxicity test data were found for unrefined / acid-treated oils. However, the potential effects of unrefined / acid treated oils can be predicted from aquatic toxicity data from studies of distillate aromatic extracts. This is valid for the following reasons: Unrefined /acid treated oils contain aromatic hydrocarbons, the quantities of which are substantially reduced through more severe refining steps. These aromatic compounds extracted from unrefined / acid treated oils are the primary constituents in distillate aromatic extracts, which are produced as by products in the refining of lubricant base oils. Therefore, aquatic toxicity data from studies of distillate aromatic extracts will provide a basis for a ‘worst case’ hazard assessment for the aromatic fraction contained in unrefined /acid treated oils.

In a read-across key semi-static 21-day long-term Daphnia magna test (OECD 211; KS=1), 10 animals/loading were exposed to the Water Accommodated Fraction (WAF) of distillate aromatic extract (PSG1860; CAS# 64742 -04 -7) at nominal concentrations of 0, 10, and 1,000 mg/L. The EL50 was > 1,000 mg/L based on reproduction. The NOEL was 1,000 mg/L based on reproduction and immobilisation (BP Oil Europe, 1995).

Supporting data estimated from the PETROTOX computer model show no long-term toxicity of unrefined / acid treated oil to aquatic invertebrates at or below its maximum attainable water solubility (Redman et al., 2010b). These data support the applied read across.