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Long-term toxicity to fish

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For unrefined / acid treated oils, the long-term toxicity for fish endpoint is addressed by read across to long-term toxicity for Daphnia. Read across to DAEs has been applied for the long-term toxicity to aquatic invertebrates endpoint, which is justified based on similarities in composition and physical/chemical properties between unrefined / acid treated oils and DAEs. Toxic effects of hydrocarbons are primarily caused by narcosis, and occur in a narrow range of molar concentrations across aquatic taxa; hence, read across between species is justified.

Supporting data estimated from the PETROTOX computer model show no long-term toxicity of unrefined / acid treated oil to freshwater fish at or below its maximum attainable water solubility (Redman et al. 2010b). These data support the applied read across.