Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The 21d EC10 reproduction of 738 µg/L for daphnia is determined in a test according to OECD TG 211 (GLP, Reliability 1). The 21d EC50 for parental mortality was 1.05 mg/L in the same test. The effect concentrations are based on active ingredient.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.738 mg/L

Additional information

Results of two daphnia reproduction tests are available. One test is performed with 3 -(Isodecyloxy) propylamine acetate (CAS no. 28701 -67 -9). The other test is performed with 3 -(Isodecyloxy) propylamine (CAS no 30113 -45 -2). The results of the first study have been read-across to the registered substance. The substances have the same chemical stucture with the only difference being the presence of acetate. Acetate is not expected to influence the ecotoxicity of the substance and this is confirmed by the derived effect concentrations for the two substances. The lowest EC10 of the two tests (3 -(Isodecyloxy) propylamine acetate)

has been selected as key value for the chemical safety assessment.

The Daphnia magna Reproduction Test with natural river water (Semi-Static, 21 d) of 3-(Isodecyloxy)propylamine acetate was conducted according to OECD 211 (Noack, 2012). 10 test organisms were used per concentration and control. At test start they were 2 to 24 hours old. Concentrations and control were analytically verified at six sampling dates with LC-MS/MS. Recoveries were 96 – 116% of the nominal concentrations in the fresh media and 88 – 103 % in the 48h old media.

The EC10-value for the reduction of the reproductive output was estimated by linear interpolation (between the highest concentration level with increased reproductive output and the following concentration level which caused a reduction of the reproductive output of 25 %) to be 0.767 mg/L (95 % confidence limits: 0.500 – 1.00 mg/L) based on the nominal test material concentration. The 21d EC10 reproduction was 0.738 mg/L (C.I. 0.481 – 1.924 mg/L) converted to active ingredient. The EC50 parental mortality was 1.05 mg/L (a.i.) determined after 21 days of exposure. The latter value is used as worst-case acute EC50 for aquatic invertebrates in the hazard assessment in the absence of reliable acute data. The effects are expressed as nominal values because the tests were performed with river water as they are intended to be used in an evaluation of the environmental risks based on the Bulk approach.