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EC number: 295-765-2 | CAS number: 92128-68-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant, guideline study, available as unpublished report, minor restrictions in design and/or reporting but otherwise adequate for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- neither S.typh TA102 nor E. coli WP2 uvrAor E.coli WP2 uvrA (pKM101) were tested.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Penta-1,3-diene
- EC Number:
- 207-995-2
- EC Name:
- Penta-1,3-diene
- Cas Number:
- 504-60-9
- IUPAC Name:
- penta-1,3-diene
- Reference substance name:
- 1,3-pentadiene
- IUPAC Name:
- 1,3-pentadiene
- Reference substance name:
- Piperylene
- IUPAC Name:
- Piperylene
- Details on test material:
- - Name of test material (as cited in study report): MRD-91-934
- Physical state: colourless liquid
- Analytical purity: considered 100%
- Lot/batch No.: 1
- Expiration date of the lot/batch: 31 May 1996
- Storage condition of test material: room temperature
Constituent 1
Constituent 2
Constituent 3
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537 and TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from Arochlor induced rat livers
- Test concentrations with justification for top dose:
- 32, 100, 320, 1000, 3200 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Remarks:
- 0.1 mL/plate DMSO
- Positive control substance:
- other: 2-aminoanthracene (2AA) 5 µg/plate (TA98, TA100, TA1535, TA1537, TA1538 + S9); N-methyl-N-nitro-N-nitrosoguanidine (MNNG) 10 µg/plate (TA100, TA1535 -S9), 9-aminoacridine (9AA) 100 µg/plate (TA1537 -S9); 2-Nitrofluorene (2NF) 5µg/plate (TA98, TA1538 -S9).
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 2 days
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: Numbers of revertant colonies per plate counted - Evaluation criteria:
- Revertant colonies counted and compared to number of revertant colonies on solvent control plates. Any value that was equal to or greater than three times the mean value of the concurrent vehicle control was considered positive.
- Statistics:
- Mean plate count and SD for each dose point was determined.
Results and discussion
Test results
- Species / strain:
- other: TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
In the initial assay neither a positive response nor a dose-related increase in revertants was observed for tester strains TA98, TA100, TA1535 and TA1538. An increase in revertant colonies which was equal to three times vehicle control was noted for TA1537 without metabolic activation at 100 µg/plate. This increase was due to the low mean value of the vehicle control. As the number of revertant colonies was identical to the non treated control this was considered not to be a positive response and was considered not to be biologically significant. Toxicity, either a reduction in the number of revertant colonies or a reduction in the background lawn, was present for all five strains.
In a repeat assay, increases were seen for tester strain TA1537 without metabolic activation at 320 µg/plate and with metabolic activation at 32 -1000 µg/plate. These apparent increases were due to the low mean value of the vehicle control. Since all test material dose data produced revertant colony counts that were no greater than the normal range of the vehicle control, these were considered not to be positive responses and were considered not to be of biological significance. A dose-related increase in revertant colonies was not observed for any of the tester strains. Toxicity, either a reduction in the number of revertant colonies or a reduction in the background lawn, was present for all five strains.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
MRD-91-934 was negative for genotoxicity in the microbial mutagenesis (Ames) assay. MRD-91-934 did not induce a significant increase in revertant colonies in any of the five tester strains with or without metabolic activation at doses up to and including 3200 µg/plate. - Executive summary:
MRD-91-934 was negative for genotoxicity in the microbial mutagenesis (Ames) assay. MRD-91-934 did not induce a significant increase in revertant colonies in any of the five tester strains with or without metabolic activation at doses up to and including 3200 µg/plate.
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