Phosphorous acid, tri-C12-14-alkyl esters

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Study generated according to valid and internationally accepted standard testing guidelines.

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Water solubility:

>= 21 - <= 24 mg/L

Temp.:

30 °C

pH:

>= 5.6 - < 8

Remarks on result:

other: the decrease of the pH solution after 24 h (from pH=8 to pH=5.6) is a strong indicator of the unstability of the substance

Details on results:

Because of the unstability of the tested substance after 24h, the water solubility study has been conducted on the 24h solution.
Using the flask method, the water solubility of the tested substance is 21 mg/l

Stability study :

The stability study of the tested substance in water is exposed in the 5.1 endpoint (hydrolysis). The study indicates that the substance degrades in a significant way beyond 24 hours, the solution becoming clouded. This degradation is certainly due to the formation of phosphorous acid by-products which can explain the reduction of the pH over time. The substance is stable during 24h in water under stirring with a concentration of 100mg/100ml. Thus, the water solubility study has to be conducted on the 24h solution.

Solubility Study :

The preliminary assays show that the solubility of the tested substance is < 1g/L.

reference solution :

	Sample (g)	Conc (g/l)	Area	Area / conc
sol 1	0,1051	0,000186868	142069	760264743
sol 2	0,1045	0,000186868	138814	742846012
			average	751555377,7
			standard deviation	1231690,3
			repeatability %	1,6

Samples solutions :

16 hours - in water
solution under stirring (Sa)			injected solutions
sample (g)	water (g)	conc (g/g)	p Sa	p thf	Conc  (g/l)	aera	aera / conc
0,988	97,324	0,0100496	10,2065	10 ml	9,118617827	171556	18814
1,0095	98,6173	0,0101328	10,0565	10 ml	9,058968742	178124	19663
1,0004	98,9307	0,0100109	9,9581	10 ml	8,862398193	138814	15663
						average	18046,6
						standard deviation	2107,2
						repeatability %	11,7
24 hours - in water
Solution sunder stirring (Sa)			injected solutions
sample (g)	water (g)	conc (g/g)	p Sa	p thf	conc (g/l)	area	area / conc
0,987	97,7519	0,0099961	10,0633	10 ml	8,942749151	122496	13698
1,0078	98,6402	0,0101136	10,1530	10 ml	9,128552437	130815	14330
1,0226	98,4267	0,0102826	10,1703	10 ml	9,296930461	168995	18178
						average	15402
						standard deviation	2424,5
						repeatabilty %	15,7

average after 24h	15401.9
average after 16h	18046.6
repeatability %	11.2

The repeatability (%) is quite lower than 15 % between the 16h- and 24h-assays.

The content in Phosphorous acid, triC12 -C14 alkyl esters is obtained by external calibration on the 16h- and 24h-solutions :

	Area/conc	ppm
average of the 24h-solutions	15401.9	21
average of the 16h-solutions	18046.6	24
reference solution	751555377,7	1000000

The content detected by chromatography analyzer on the 24h-solutions in water is 21 mg/L

Thus, we can assume that the water solubility of the tested substance is 21 mg/L

The phosphorous acid, triC12 -C14 alkyl esters is stable 24h in water at a concentration of 100 mg/10 ml.

Conclusions:

Interpretation of results: slightly soluble (0.1-100 mg/L)
With the flask method, the solubility of the phosphorous acid, triC12 -C14 alkyl esters was 21 mg/L.

Executive summary:

The water solubility of the phosphorous acid, triC12-C14 alkylesters was investigated in a GLP-compliant study performed in accordance with EC A.6 method and OECD Guideline No. 105, using Flask method.

The solubility of the phosphorous acid, triC12 -C14 alkyl esters was 21 mg/L.

The phosphorous acid, triC12 -C14 alkyl esters is stable 24h in water at a concentration of 100 mg/10 ml.

Description of key information

The water solubility of the phosphorous acid, triC12-C14 alkyl esters was investigated in a GLP-compliant study performed in accordance with EC A.6 method and OECD Guideline No. 105, using Flask method.

The solubility of the phosphorous acid, triC12 -C14 alkyl esters was 21 mg/L.

The phosphorous acid, triC12 -C14 alkyl esters is stable 24h in water at a concentration of 100 mg/10 ml.

Key value for chemical safety assessment

Water solubility:

21 mg/L

at the temperature of:

30 °C

Additional information

The water solubility of the phosphorous acid, triC12-C14 alkylesters was investigated in one GLP-compliant study performed in accordance with standard methods, without deviations. The study is considered as reliable (Klimisch 1) and is selected as a key study for the endpoint.