2-Cyclohexen-1-one, 3,4-dihydroxy-2,6,6-trimethyl-, sodium salt (1:1)

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed in accordance with OECD Guideline and compliant with GLP criteria. Minor restrictions in reporting, but otherwise considered adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1184842-87-2

Cas Number:

1184842-87-2

IUPAC Name:

1184842-87-2

Details on test material:

- Substance name: C9-Diol Na-Salz

Sampling and analysis

Analytical monitoring:

not required

Test solutions

Details on test solutions:

- Stock solution: 2000 mg/L

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Municipal activated sludge from laboratory units 1 u. 3, which are fed with municipal sewage. Activated sludge concentrationis 1 g/L based on dry substance

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test conditions

Nominal and measured concentrations:

- Nominal concentrations: 0 (control) and 1000 mg/L.

Reference substance (positive control):

yes

Remarks:

3.5-Dichlorphenol

Results and discussion

Effect concentrations

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

An increase rather than an inhibition of respiration was observed at the test concentration of 1000 mg/L. The test substance is an aqueous solution with about 20 - 30% active incredient. Based on the active incredient and as a conservative approach, the 16h EC10 is determined at >200 mg/L.

Results with reference substance (positive control):

- EC20 = ca. 1.5 mg/L
- EC50 = ca. 6.5 mg/L