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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed in accordance with OECD Guideline and compliant with GLP criteria. Minor restrictions in reporting, but otherwise considered adequate for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Substance name: C9-Diol Na-Salz

Sampling and analysis

Analytical monitoring:
not required

Test solutions

Details on test solutions:
- Stock solution: 2000 mg/L

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Municipal activated sludge from laboratory units 1 u. 3, which are fed with municipal sewage. Activated sludge concentrationis 1 g/L based on dry substance

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Nominal and measured concentrations:
- Nominal concentrations: 0 (control) and 1000 mg/L.
Reference substance (positive control):
yes
Remarks:
3.5-Dichlorphenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
An increase rather than an inhibition of respiration was observed at the test concentration of 1000 mg/L. The test substance is an aqueous solution with about 20 - 30% active incredient. Based on the active incredient and as a conservative approach, the 16h EC10 is determined at >200 mg/L.
Results with reference substance (positive control):
- EC20 = ca. 1.5 mg/L
- EC50 = ca. 6.5 mg/L

Applicant's summary and conclusion