Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed in accordance with EU Guideline and compliant with GLP criteria.

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:

Test material

Constituent 1
Reference substance name:
Cas Number:
Details on test material:
- Name of test substance: Asta-C9-Diol-Na-Salt in water
- Physical state/appearance: Liquid, yellow
- Storage conditions: At about 4 °C, exclusion of light
- Homogeneity: Homogeneous

Sampling and analysis

Analytical monitoring:
Details on sampling:
For standard solutions the reference standard was dissolved and diluted in demin. water. Depending on concentration the samples were diluted
using carrier prior the injection.

Test solutions

Details on test solutions:
Separately for each concentration 0.50, 1.01, 50.00, 100.00 g test substance were added to the test water using an ultra-turrax stirrer. From the beginning of preparing the test solutions until termination of placing the fish into the aquaria 20 minutes were needed.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Common name: Zebra fish
- Strain: HAM. and BUCH.
- Animal breeder/suppijer: Charles River Aquatics, Someren; Netherlands
- Hatching date: Sep. 03, 1996
- Age of the animais: About 8 months
- Weight: 0.29 (0.18 - 0.48) g
- Length: 3.3 (2.8 - 4.0) cm
- Physical conditions: no abnormalities
- Housing: The fish were kept in a flow-through tank in tap water, not chlorinated, passed through a charcoal filter and aerated with oil-free air.
- Duration of housing: About 2 months
- Water temperature: About 21-23 °C
- Mortality: During the last 14 days: 0.4 Z
- Medical treatment: none
- Diet: "TETRA MIN" standard feed for aquarium fish TETRA - WERKE, MELLE, FRG, ad libitum; and on workdays generally additionally live artemia.

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
96 h

Test conditions

250 mg/L as CaCO3
Test temperature:
22 °C
Dissolved oxygen:
6.0-9.0 mg/L
Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 50, 100, 5000 and 10000 mg/L (based on range finding study).
- Measured concentrations (t=1h): <5, 48, 91, 4900 and 9400 mg/L
- Measured concentrations (t=96h): <5, 49, 95, 4800 and 10000 mg/L
Details on test conditions:
- Test vessels: All-glass aquaria (30 x 22 x 24 cm), one per each test group
- Test water: Municipal water of the City of Frankenthal, not chlorinated and passed through a charcoal filter. The test water is regularly assayed for chemical contaminants by the municipal authorities of Frankenthal and the technical services of BASF Aktiengesellschaft as well as for presence of microbes by a contract laboratory.
- Volume of water: 10 liters (water depth about 17 cm).
- Aeration: Slight
- Photoperiod: 16 hours light and 8 hours darkness
- No. of animals per vessel: 10
- Adaptation: Test conditions corresponded to housing conditions.
- Withdrawal of food: 1 day before and during exposure

Determination or calculation of the median lethal concentration (LC50), the NOEC and the symptoms of the noC95 for the calculations using the probit analysis
Reference substance (positive control):

Results and discussion

Effect concentrations
96 h
Dose descriptor:
Effect conc.:
> 2 940 mg/L
Nominal / measured:
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Details on results:
First at the concentration of 10000 mg/L did mortality occur (5%). During the study, at 10000 mg/L, was slight to very slight apathy noted. Concentrations given here are based on test substance. Whereas based on active incredient, 5% mortality occured at 2940mg/L.
Reported statistics and error estimates:
Finney, D.J., Probit Analysis, Cambr. Univ. Press, 3rd ed., 1971; certain aspects of this method have been modified.

Applicant's summary and conclusion