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Long-term toxicity to aquatic invertebrates

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Description of key information

Based on long-term (chronic) toxicity study data, the product is very likely not harmful to aquatic invertebrates. 

Key value for chemical safety assessment

Additional information

The long-term toxicity to aquatic invertebrates was assessed with the test item in a key study according to OECD 211 (2012). In this semi-static reproduction test, young Daphnia magna (< 24 h old) were exposed to the test item for 21 days.

The test item is poorly water soluble, therefore a limit test with a single concentration at the limit of solubility was performed. The limit of solubility was reached by applying the Water Accommodated Fraction (WAF) method prepared from a loading of 10 mg/L with sonication, heating, prolonged mixing and sequential filtration to separate the not dissolved test item. The aim of a limit test was to demonstrate that the LOEC and NOEC values based on number of alive offspring, immobilization and final body size (body length) of parent daphnid were respectively higher than and higher than or equal to the solubility limit of the test item in test medium. The test was performed in glass beakers of 150 mL capacity, containing 100 mL of each treatment per replicate and a complete renewal three times per week. At each renewal the parent daphnids were transferred from old to fresh solutions. The offspring produced in each test vessel was counted and removed daily. The observations on immobilization of parent animals were reported daily. The body size of parent daphnids was measured at exposure termination.

 

Analytical measurements of the test item concentration were carried out by measuring the total organic carbon (TOC) content.

The results of the limit semi-static test demonstrated no effects on reproductive output (the mean cumulative number of offspring per survivor), immobilization, final body size of parent daphnid and intrinsic rate of population growth and the age of the first reproduction; after 21 days of exposure in the WAF of a loading of 10 mg/L. Since no immobilization or any other effects on parent daphnids were observed, the conclusions of the estimations based on the number of living offspring produced per parent Daphnia alive at exposure termination (i.e. the mean cumulative number of offspring per survivor) are the same as the conclusions of the estimations based on the total number of living offspring produced per parent Daphnia introduced at exposure initiation (excluding parental accidental and/or inadvertent immobilization from the analysis). The endpoint values based on the number of living offspring produced per parent Daphnia alive at exposure termination, immobilization of parent daphnid, the intrinsic rate of population growth, the final body size (body length) of parent daphnid and the age of the first reproduction are up to the solubility limit of the test item in test medium, i.e. up to a loading rate of 10 mg/L. Therefore following overall effect concentrations (mg/L) were obtained based on the WAF of a loading of 10 mg/L: LOEC > 10 mg/L, NOEC ≥ 10 mg/L, 21 d-EC10 > 10 mg/L and 21-d EC50 > 10 mg/L.