Vanadium trichloride oxide

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non standard protocol, but well described method

Data source

Reference

Reference Type:

publication

Title:

Effect of 3,4-Dichloroaniline and Metavanadate on Daphnia Populations.

Author:

Nelly Van Der Hoeven

Year:

1989

Bibliographic source:

Ecotoxicology and Environmental Safety 20:53-70 (1990)

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

A Daphnia magna population was exposed to metavanadate for up to 14 weeks, under continuous flowthrough conditions. The population size, length and eppiphia production were observed.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

metavanadate

IUPAC Name:

metavanadate

Details on test material:

no detail on test material was described

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
vanadate: RNCAS: 13981-20-9
MW: 98.9
formula: VO3-

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

The medium was groundwater out of the neighborhood of Linschoten, obtained directly from a water company. The mineral composition was adjusted to a standard:
Na(+) = 27,4 mg/L
K (+) = 7,8 mg/L
Ca (2+) = 54,4 mg/L
Mg (2+) = 17,5 mg/L
Cl (-) = 96,6 mg/L
SO4 (2-) = 70,1 mg/L
HCO3 (-) = 84,8 mg/L
pH: 8.1

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

All experiments were started with 5 egg-bearing females D. magna, 10 days old.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

14 wk

Test conditions

Test temperature:

22°C

pH:

8.1

Nominal and measured concentrations:

0.56, 1 and 1.8 mg V/L

Details on test conditions:

TEST SYSTEM

- Material, size, headspace, fill volume: 2L conical flasks, volume of medium: 800mL
- Aeration: constant, slow
- Renewal rate of test solution: flow rate: constant at 1 liter/ day
vessels cleaned and refilled on Mondays, Wednesdays and Fridays. On the other days they were filled with fresh medium.
The inflow medium contained 400.000.000 C. pyrenoidosa cells and 500.000.000 yeast cells per liter.
- Photoperiod: 14 h light/ 10 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : population count, mean lenght, ephippial eggs count, every week

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 wk

Dose descriptor:

NOEC

Effect conc.:

0.56 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Remarks:

vanadate or vanadium element

Basis for effect:

reproduction

Effect concentrations 2

Duration:

14 wk

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Remarks:

vanadate (or vanadium element)

Basis for effect:

mortality

Reported statistics and error estimates:

no data

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Executive summary:

A population of Daphnia magna was exposed to metavanadate during 14 weeks under continuous flow through conditions. Three concentrations of the toxicant were used: 0.56, 1.0 and 1.8 mg/L. Effects of metavanadate were observed every week: population dynamic, mean lenght of daphnids and production of eggs. The test was carried out under standard protocol, but the methodology and the dilution medium were well described.

A 14 weeks NOEC value of 0.56 mg V/L was shown for the effect of metavanadate on ephippia production and a NOEC of 1.0 mg V/ L was observed for mortality (promotion effect up this concentration).