1-Propene, hydroformylation products, high-boiling

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study incl. analytics

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Oxooel 740
- Physical state: Liquid/ yellowish, clear
- Analytical purity: Sum of identified hydroformylation products: 85.4 cor.-area-% (see analytical report 09L00241)
- Lot/batch No.: TK285_20090716
- Expiration date of the lot/batch: 2010-07-16
- Stability under test conditions: stable under storage conditions over the exposure period.
- Storage condition of test material: The test substance was stored in a refrigerator, dry storage.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Samples for concentration control analysis were taken from each freshly prepared solution (0 h and 24 h) in additionally prepared vessels without daphnids and at the end of each exposure interval (after approx. 24 h and 48 h) from all test vessels with mobile daphnids and the lowest concentration with 100% immobilisation. The samples were transported to the Analytical Laboratory on the day of sampling.
-Sample storage conditions before analysis: Storage at room temperature

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

The test substance was homogenized by shaking the container before use and all test solutions were prepared individually (differential loading). The test substance was weighed into a 1 L volumetric flask and filled to the mark with test medium. The solutions were then stirred for approximately 30 min at 20 ± 2 °C and visually checked for any undissolved test substance. Fresh test solutions were prepared and renewed daily and the test was performed in closed test vessels to maximize the exposure to all soluble components of the test substance. All test solutions were visibly clear over the exposure period

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978.
From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen
- Age at study initiation: < 24 hours( at least 3rd brood progeny)
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.42 mmol/L

Test temperature:

19.5-19.9 °C

pH:

8.0-8.3

Dissolved oxygen:

7.7-8.7

Nominal and measured concentrations:

-nominal: 4.6, 10, 22, 46, and 100 mg/L
-measured: 4.4, 9.6, 21.3, 45.0, and 101 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) sealed with gas impermeable teflon caps (nominal volume 23 mL)
- Type: Due to the volatility of the test substance, this study was conducted as a static-renewal in a closed system. The renewal period was 24 hours

- Test volume: approx. 23 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.22 animals/mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium with the properties:
- Total hardness: 2.20 – 3.20 mmol/L
- Acid capacity up to pH 4.3: 0.80 – 1.00 mmol/L
- Molar ratio Ca:Mg: about 4 : 1
- pH value: 7.5 – 8.5
- Conductivity: 550 - 650 μS/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: About 20 – 850 lux at a wave length of 400 -750 nm

EFFECT PARAMETERS MEASURED :
-Observations: mobile daphnids after 24 and 48 h
-Measurements: Temperature, pH and oxygen content after 0 and 48 h

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

63.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL = 51.3-80.9 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes