3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxylic acid

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): the test substance was solubilised in acetone. The solution was mixed with quartz sand and subsequently air dried. The quartz sand was mixed with artificial soil.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Source: bred at testing facility
- Age at test initiation: < 1a with clitellum
- Weight at test initiation (range): 300-500 mg

ACCLIMATION
- Acclimation period: 1 day
- Acclimation conditions (same as test or not): same as test conditions

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

56 d

Test conditions

Test temperature:

19- 21 °C

pH:

5.3 - 5.9 (test start)
6.2 - 6.8 (test termination)

Moisture:

60 % of WHC

Details on test conditions:

TEST SYSTEM
- Test container (material, size): plastic dishes (13.5*6 cm)
- Amount of soil or substrate: 600 g soil (dry weight)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 5 % Sphagnum-peat, 20 % Kaolin clay, 75 % quartz sand
- Maximum water holding capacity (in % dry weigth): 58 % of dry weight

OTHER TEST CONDITIONS16:8 h day-night regime
- Photoperiod: 16:8 h day-night regime
- Light intensity: 430 - 630 lux

EFFECT PARAMETERS MEASURED:
Mortality, body weight, feeding activity and reproduction

Nominal and measured concentrations:

Nominal test concentrations:
control, 0.33, 0.67, 1.33, 2.67 and 5.33 mg/kg dw soil

Reference substance (positive control):

yes

Remarks:

Benlate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

56 d

Dose descriptor:

NOEC

Effect conc.:

>= 5.33 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

56 d

Dose descriptor:

EC50

Effect conc.:

> 5.33 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

No mortality observed. During the testing period the earthworms gained weight compared to the control group. Effects on the feeding activity were not observed.

Results with reference substance (positive control):

Benlate had no effect on earthworm survival but reduced the weight gain and the feeding activity of the animals. The reproduction rate was statistically significant inhibited.