Alcohols, C12-18, ethoxylated

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

05 JAN - 24 JAN 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Alcohols, C12-14, ethoxylated

EC Number:

500-213-3

EC Name:

Alcohols, C12-14, ethoxylated

Cas Number:

68439-50-9

IUPAC Name:

68439-50-9

Details on test material:

- Substance type: C12-C14 Fatty alkoholpolyethylenglycolether with 2 EO
- Physical state: liquid
- Analytical purity: 73% a.s. (w/w)
- Lot/batch No.: Pt. 24, Tank 7

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): Stock solution (0.23 g substance in 20 g quarz sand) was mixed with 1680 g steril substrate to a concentration of 100 mg/kg dry substrate.

Test organisms

1

Species:

Triticum aestivum

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: wheat
- Plant family: Poacea

2

Species:

Brassica alba

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: White mustard
- Plant family: Brassicaceae

3

Species:

Lepidum sativum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Garden cress
- Plant family: Brassicaceae

Study design

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

yes

Total exposure duration:

19 d

Test conditions

Test temperature:

20 - 24 °C

pH:

5.0 - 7.5

Moisture:

approximately 60%

Details on test conditions:

TEST SYSTEM
- Test container (type, material, size): plastic pots, diameter 6 cm (bottom) and 8 cm (top), 6 cm (height)
- Amount of soil: approximately 100 g substrate
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 4



SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: LUFA soil Speyer (Sp. 2.3)
- Soil texture (if natural soil): particle < 20 µm: 10 - 20%
- Organic carbon (%): < 1.5%



GROWTH CONDITIONS
- Photoperiod: 16 h day / 8 h night
- Light intensity and quality: day: 1500 - 2000 lux, night: approximately 50 lux
- Day/night temperatures: 20 - 24 °C
- Relative humidity (%): 90% - 100%
- Interval of applications: 1 - 2 times per week

Nominal and measured concentrations:

100 mg/kg soil

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

Triticum aestivum

Duration:

19 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks:

and growth

Effect concentrations 2

Species:

Brassica alba

Duration:

19 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks:

and growth

Effect concentrations 3

Species:

Lepidum sativum

Duration:

19 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks:

and growth

Details on results:

SEEDLING EMERGENCE
- Percent seedling emergence: Triticum aestivum: 100 mg/kg: 90% (85% control), Brassica alba: 100 mg/kg: 80% (80% control), Lepidum sativum: 100 mg/kg: 95% (control: 95%)
- Fresh weight: Triticum aestivum: 100 mg/kg: 357 mg (389 mg control), Brassica alba: 100 mg/kg: 248 mg (228 mg control), Lepidum sativum: 100 mg/kg: 71 mg (control: 61 mg)