Alcohols, C12-18, ethoxylated

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Fish were exposed in a flow-through system to the test substance during an uptake phase of up to 72 h. The following elimination phase lasted for 24 h.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

C12EO8

IUPAC Name:

C12EO8

Constituent 2

Reference substance name:

C14EO4

IUPAC Name:

C14EO4

Constituent 3

Reference substance name:

C16EO8

IUPAC Name:

C16EO8

Details on test material:

- Name of test material (as cited in study report): C12EO8, C14EO4, C16EO8
- Analytical purity: > 97%

Radiolabelling:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnow
- Source: hatchery of Utrecht University, The Netherlands
- Weight at study initiation (mean and range, SD): 0.5 - 1.0 g

ACCLIMATION
- Acclimation period: at least 1 week
- Type and amount of food: Lapis dry feed at a rate of 1% of body weight
- Feeding frequency: daily

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

54 - 72 h

Total depuration duration:

24 h

Test conditions

Test temperature:

20.7 - 22.5 °C

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 - 8 L aquaria
- No. of organisms per vessel: 30


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater was prepared by dissolving 0.75 mM CaCl2 x 2H2O, 0.46 mM MgSO4 x 7H2O, 1.51 mM NaHCO3, 0.04 mM KH2PO4, 1.01 mM NaNO3 and 0.10 mM Na2SiO3 in distilled water.

Nominal and measured concentrations:

The AE concentrations were higher than the critical micelle concentration. 0.029 µM steady state concentration of the test compound in water

Reference substance (positive control):

no

Results and discussion

Lipid content

Lipid content:

0.9 - 8.2 %

Time point:

start of exposure

Remarks on result:

other: average content: 4.9%

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

12.7 L/kg

Basis:

not specified

Time of plateau:

24 h

Calculation basis:

steady state

Remarks on result:

other: for C12EO8

Remarks:

Conc.in environment / dose:0.570 µM steady state concentration of the test compound in water

Bioaccumulation factor 2

Type:

BCF

Value:

237 L/kg

Basis:

not specified

Time of plateau:

24 h

Calculation basis:

steady state

Remarks on result:

other: for C14EO4

Remarks:

Conc.in environment / dose:0.029 µM steady state concentration of the test compound in water

Bioaccumulation factor 3

Type:

BCF

Value:

387.5 L/kg

Basis:

not specified

Time of plateau:

24 h

Calculation basis:

steady state

Remarks on result:

other: for C16EO8

Remarks:

Conc.in environment / dose:0.033 µM steady state concentration of the test compound in water

Details on results:

- Mortality of test organisms: no mortality was observed
- Behavioural abnormalities: no signs of sublethal effects, such as swimming or breathing behavior, were observed during the experiments.

Any other information on results incl. tables

The test substance was not stored inside fish and no relationship between BCF and lipid content was observed. The elimination rate suggested rapid biotransformation of the test substance.